Rad54B Double Nickase Plasmid (h): sc-403794-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rad54B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rad54B Double Nickase Plasmid (h) and Rad54B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAD54B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rad54B Antibody (19-K2): sc-101234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad54B Double Nickase Plasmid (h) | sc-403794-NIC | 20 µg | $410.00 | |||
Rad54B Double Nickase Plasmid (h2) | sc-403794-NIC-2 | 20 µg | $410.00 |
RAD54B encodes Rad54B, a SWI2/SNF2-family DNA-dependent ATPase that functions in homologous recombination by cooperating with RAD51 to remodel DNA and promote strand invasion during repair of double-strand breaks. Rad54B contributes to genome stability through roles in DNA damage response signaling, replication-associated repair, and recovery from genotoxic stress, linking it to core homologous recombination and recombination-mediated checkpoint pathways. Altered RAD54B activity can increase chromosomal instability and mutational burden, features frequently studied in the context of tumor biology and DNA repair–defective states. Accordingly, RAD54B is widely investigated for its impact on recombination efficiency, sensitivity to DNA damaging agents, and mechanisms governing faithful chromosome maintenance.
Rad54B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAD54B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAD54B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAD54B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAD54B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.