Rad54 Antibody (A-4): sc-166370
- Rad54 Antibody (A-4) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-152 mapping at the N-terminus of Rad54 of human origin
- recommended for detection of Rad54 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Rad54 Antibody (A-4) for WB applications. This reagent is now offered in a bundle with Rad54 Antibody (A-4) (see ordering information below).
Rad54 Antibody (A-4) is a mouse monoclonal IgG1 antibody that detects Rad54 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad54 plays a crucial role in DNA repair processes, particularly in homologous recombination, by facilitating the pairing of homologous DNA strands, which is essential for maintaining genomic stability. Rad54 is part of the DEAD-like helicase superfamily and binds to double-stranded DNA, inducing topological changes that promote effective DNA recombination. Rad54 is abundant in tissues such as the testis and spleen, where rapid cell division occurs, and contributes to critical cellular processes. Mutations in the RAD54 gene have been linked to various cancers, including primary lymphoma and colon cancer, highlighting RAD54′s significance in tumorigenesis and genomic integrity maintenance. anti-Rad54 antibody (A-4) serves as an invaluable tool for researchers studying DNA repair mechanisms and RAD54′s implications in cancer biology.
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Rad54 Antibody (A-4) References:
- ATP-dependent and independent functions of Rad54 in genome maintenance. | Agarwal, S., et al. 2011. J Cell Biol. 192: 735-50. PMID: 21357745
- Analysis of the activities of RAD54, a SWI2/SNF2 protein, using a specific small-molecule inhibitor. | Deakyne, JS., et al. 2013. J Biol Chem. 288: 31567-80. PMID: 24043618
- Mechanistic basis for increased human gene targeting by promoterless vectors-roles of homology arms and Rad54 paralogs. | Saito, S., et al. 2017. FEBS J. 284: 2748-2763. PMID: 28622436
- RAD54 N-terminal domain is a DNA sensor that couples ATP hydrolysis with branch migration of Holliday junctions. | Goyal, N., et al. 2018. Nat Commun. 9: 34. PMID: 29295984
- Rad54/Rad54B deficiency is associated to increased chromosome breakage in mouse spermatocytes. | Russo, A., et al. 2018. Mutagenesis. 33: 323-332. PMID: 30204892
- RAD54 promotes alternative lengthening of telomeres by mediating branch migration. | Mason-Osann, E., et al. 2020. EMBO Rep. 21: e49495. PMID: 32337843
- Rad54 Drives ATP Hydrolysis-Dependent DNA Sequence Alignment during Homologous Recombination. | Crickard, JB., et al. 2020. Cell. 181: 1380-1394.e18. PMID: 32502392
- Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex. | Crickard, JB., et al. 2020. EMBO J. 39: e105705. PMID: 32790929
- NUCKS1 promotes RAD54 activity in homologous recombination DNA repair. | Maranon, DG., et al. 2020. J Cell Biol. 219: PMID: 32876692
- Critical roles of Rad54 in tolerance to apigenin-induced Top1-mediated DNA damage. | Zhao, Z., et al. 2021. Exp Ther Med. 21: 505. PMID: 33791014
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad54 Antibody (A-4) | sc-166370 | 200 µg/ml | $316.00 | |||
Rad54 Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-542661 | 200 µg Ab; 20 µg BP | $354.00 |