Rad53 Antibody (B-6): sc-74426
- Rad53 Antibody (B-6) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1-300 of Rad53 of Saccharomyces cerevisiae origin
- recommended for detection of Rad53 of y origin by WB, IP and ELISA
- m-IgG2a BP-HRP is the preferred secondary detection reagent for Rad53 Antibody (B-6) for WB applications. This reagent is now offered in a bundle with Rad53 Antibody (B-6) (see ordering information below).
Rad53 Antibody (B-6) is a mouse monoclonal IgG2a antibody that detects Rad53 in yeast by applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Rad53 is a crucial protein kinase that plays a vital role in the cellular response to DNA damage, particularly during the G2 and S phase checkpoints of the cell cycle. Rad53 activation is essential for halting cell cycle progression, allowing for the repair of damaged DNA before replication occurs, thereby maintaining genomic integrity. Rad53 interacts with several key proteins, including Mec1, which is a homolog of the human ATM protein and is responsible for initiating the DNA damage response. The interaction between Rad53 and Mec1 is critical, as this ensures that the cell can effectively respond to DNA damage signals and prevent the propagation of mutations. Additionally, Rad53 is involved in regulating other checkpoint proteins such as Pds1 and Mad2, which are essential for monitoring spindle integrity and ensuring proper chromosome segregation. Rad53′s ability to coordinate these processes underscores its importance in cellular health and the prevention of cancerous transformations, making anti-Rad53 antibody (B-6) an invaluable tool for researchers studying DNA damage response mechanisms in yeast.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rad53 Antibody (B-6) References:
- Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling. | Lee, SJ., et al. 2003. Mol Cell Biol. 23: 6300-14. PMID: 12917350
- The Rad53 signal transduction pathway: Replication fork stabilization, DNA repair, and adaptation. | Branzei, D. and Foiani, M. 2006. Exp Cell Res. 312: 2654-9. PMID: 16859682
- Regulation of the Rad53 protein kinase in signal amplification by oligomer assembly and disassembly. | Jia-Lin Ma, N. and Stern, DF. 2008. Cell Cycle. 7: 808-17. PMID: 18239457
- Structural basis of Rad53 kinase activation by dimerization and activation segment exchange. | Wybenga-Groot, LE., et al. 2014. Cell Signal. 26: 1825-36. PMID: 24815189
- The Rad53CHK1/CHK2-Spt21NPAT and Tel1ATM axes couple glucose tolerance to histone dosage and subtelomeric silencing. | Bruhn, C., et al. 2020. Nat Commun. 11: 4154. PMID: 32814778
- Rad53 checkpoint kinase regulation of DNA replication fork rate via Mrc1 phosphorylation. | McClure, AW. and Diffley, JF. 2021. Elife. 10: PMID: 34387546
- Yeast Stn1 promotes MCM to circumvent Rad53 control of the S phase checkpoint. | Gasparayan, H., et al. 2022. Curr Genet. 68: 165-179. PMID: 35150303
- Rad53 arrests leading and lagging strand DNA synthesis via distinct mechanisms in response to DNA replication stress. | He, R. and Zhang, Z. 2022. Bioessays. 44: e2200061. PMID: 35778827
- Prevalent and dynamic binding of the cell cycle checkpoint kinase Rad53 to gene promoters. | Sheu, YJ., et al. 2022. Elife. 11: PMID: 36520028
- Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates. | Hu, J., et al. 2023. Nucleic Acids Res. 51: 11688-11705. PMID: 37850655
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad53 Antibody (B-6) | sc-74426 | 200 µg/ml | $316.00 | |||
Rad53 Antibody (B-6): m-IgG2a BP-HRP Bundle | sc-546010 | 200 µg Ab; 10 µg BP | $354.00 |