Rad53 Antibody (A-9): sc-74427
- Rad53 Antibody (A-9) is a mouse monoclonal IgG1 κ Rad53 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-300 of Rad53 of Saccharomyces cerevisiae origin
- Rad53 Antibody (A-9) is recommended for detection of Rad53 of y origin by WB, IP and ELISA
- Anti-Rad53 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rad53 Antibody (A-9). This work is in progress.
Rad53 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rad53 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-Rad53 antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad53 plays a critical role in the DNA damage response, particularly in the regulation of cell cycle checkpoints, which are essential for maintaining genomic integrity. Rad53 (A-9) monoclonal antibody functions as a key protein kinase that mediates the G2 and S phase arrest in response to DNA damage, allowing cells to repair their DNA before proceeding with division. Rad53 activation is intricately regulated by Mec1, a homolog of the human ATM protein, which senses DNA damage and initiates the checkpoint signaling cascade. Rad53 interacts with several other proteins, including Rad9, Rad17, Rad24, and Mec3, which are involved in the activation of the G1 and G2 checkpoints, as well as Pol2, which is crucial for the S phase checkpoint. This coordinated response underscores Rad53′s importance in preventing the propagation of damaged DNA, thereby playing a vital role in cancer prevention and cellular health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rad53 Antibody (A-9) References:
- Checkpoint Kinase Rad53 Couples Leading- and Lagging-Strand DNA Synthesis under Replication Stress. | Gan, H., et al. 2017. Mol Cell. 68: 446-455.e3. PMID: 29033319
- Rad53- and Chk1-Dependent DNA Damage Response Pathways Cooperatively Promote Fungal Pathogenesis and Modulate Antifungal Drug Susceptibility. | Jung, KW., et al. 2019. mBio. 10: PMID: 30602579
- Mec1-independent activation of the Rad53 checkpoint kinase revealed by quantitative analysis of protein localization dynamics. | Ho, B., et al. 2023. Elife. 12: PMID: 37278514
- The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase. | Li, R., et al. 1993. Nature. 366: 82-4. PMID: 8232541
- DUN1 encodes a protein kinase that controls the DNA damage response in yeast. | Zhou, Z. and Elledge, SJ. 1993. Cell. 75: 1119-27. PMID: 8261511
- Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways. | Sanchez, Y., et al. 1996. Science. 271: 357-60. PMID: 8553072
- Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s). | Yamamoto, A., et al. 1996. J Cell Biol. 133: 99-110. PMID: 8601617
- Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae. | Siede, W., et al. 1996. Nucleic Acids Res. 24: 1669-75. PMID: 8649984
- Yeast pip3/mec3 mutants fail to delay entry into S phase and to slow DNA replication in response to DNA damage, and they define a functional link between Mec3 and DNA primase. | Longhese, MP., et al. 1996. Mol Cell Biol. 16: 3235-44. PMID: 8668138
- A novel role for the budding yeast RAD9 checkpoint gene in DNA damage-dependent transcription. | Aboussekhra, A., et al. 1996. EMBO J. 15: 3912-22. PMID: 8670896
- A meiotic recombination checkpoint controlled by mitotic checkpoint genes. | Lydall, D., et al. 1996. Nature. 383: 840-3. PMID: 8893012
- RAD9 and DNA polymerase epsilon form parallel sensory branches for transducing the DNA damage checkpoint signal in Saccharomyces cerevisiae. | Navas, TA., et al. 1996. Genes Dev. 10: 2632-43. PMID: 8895664
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad53 Antibody (A-9) | sc-74427 | 200 µg/ml | $322.00 | |||
Rad53 Antibody (A-9) AC | sc-74427 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rad53 Antibody (A-9) HRP | sc-74427 HRP | 200 µg/ml | $322.00 | |||
Rad53 Antibody (A-9) FITC | sc-74427 FITC | 200 µg/ml | $336.00 | |||
Rad53 Antibody (A-9) PE | sc-74427 PE | 200 µg/ml | $349.00 | |||
Rad53 Antibody (A-9) Alexa Fluor® 488 | sc-74427 AF488 | 200 µg/ml | $364.00 | |||
Rad53 Antibody (A-9) Alexa Fluor® 546 | sc-74427 AF546 | 200 µg/ml | $364.00 | |||
Rad53 Antibody (A-9) Alexa Fluor® 594 | sc-74427 AF594 | 200 µg/ml | $364.00 | |||
Rad53 Antibody (A-9) Alexa Fluor® 647 | sc-74427 AF647 | 200 µg/ml | $364.00 | |||
Rad53 Antibody (A-9) Alexa Fluor® 680 | sc-74427 AF680 | 200 µg/ml | $364.00 | |||
Rad53 Antibody (A-9) Alexa Fluor® 790 | sc-74427 AF790 | 200 µg/ml | $364.00 |