Rad51B Antibody (F-12): sc-377192

Rad51B Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody designed to target an internal epitope encompassing amino acids 141-255 of human Rad51B, ensuring precise detection in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad51B, also called R51H2, mREC2, and Rad51l1, is a vital member of the Rad51 paralog family, playing a critical role in the homologous recombination repair pathway that is essential for accurate repair of DNA double-strand breaks and maintenance of genomic stability. Rad51B forms part of the BCDX2 complex, which includes Rad51C, Rad51D, and XRCC2, facilitating formation and stabilization of RAD51 nucleoprotein filament necessary for effective DNA repair. This complex interacts closely with key proteins such as BRCA1 and BRCA2, which are integral to DNA damage response and repair mechanisms, thereby enhancing cellular ability to maintain genomic integrity and prevent mutations that could lead to cancer. Rad51B interacts with Rad52, further promoting homologous recombination by aiding in annealing of complementary DNA strands. These protein-protein interactions regulate Rad51B′s activity and localization within cells, ensuring timely and accurate response to DNA damage. Rad51B monoclonal antibody (F-12) exhibits high specificity with no cross-reactivity against other Rad51 family members, providing reliable results for researchers investigating DNA repair pathways, cancer biology, and genomic integrity. Available in both non-conjugated form and various conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-Rad51B antibody (F-12) offers versatile applications to meet diverse experimental needs.

The preferred Rad51B antibody is Rad51B Antibody (1H3/13): sc-53430.