Rad51B Antibody (1H3/13): sc-53430

Rad51B Antibody (1H3/13) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect human Rad51B protein with exceptional accuracy in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Rad51B monoclonal antibody (1H3/13) is raised against a His-tagged recombinant full-length human Rad51B protein, ensuring robust and specific binding to the target antigen. Rad51B, also called R51H2, mREC2, and Rad51l1, is a crucial member of the Rad52 family involved in homologous recombination repair (HRR) of DNA double-strand breaks, a vital process for maintaining genomic stability and preventing mutations that can lead to cancerous transformations. Structurally, Rad51B contains distinct domains facilitating interaction with other Rad51 paralogs, such as Rad51C, Rad51D, and XRCC2, forming multi-protein complexes essential for accurate repair of DNA damage. These structural features enable Rad51B to participate in homology search and DNA strand pairing, which are critical steps in DNA repair. Anti-Rad51B antibody (1H3/13) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes, providing versatile options for diverse experimental setups. Rad51B (1H3/13) antibody helps researchers gain deeper insights into structural and functional mechanisms of DNA repair, maintaining genomic integrity, and implications in cancer development and treatment, making this tool essential for advanced biomedical research and diagnostics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rad51B Antibody (1H3/13) References:

1. Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts.  |  Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
2. Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre.  |  Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
3. In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.  |  Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
4. In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.  |  Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
5. The human Rap1 protein complex and modulation of telomere length.  |  O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
6. DNA annealing mediated by Rad52 and Rad59 proteins.  |  Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
7. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.  |  Bekker-Jensen, S., et al. 2006. J Cell Biol. 173: 195-206. PMID: 16618811
8. Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae.  |  Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad51B Antibody (1H3/13) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad51B Antibody (1H3/13) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad51B Antibody (1H3/13) Specifications

Alternate Product Name: Rad51B

Product Clone Name: 1H3/13

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Rad51B

Epitope Species: human

Product Reactivity/Detection: Rad51B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Rad51B Antibody (1H3/13): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin