Rad23B Antibody (H-8): sc-137088

Rad23B Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rad23B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rad23B antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad23B protein plays a critical role in nucleotide excision repair (NER), a vital cellular process that corrects DNA damage caused by ultraviolet light and other environmental factors. Rad23B protein is predominantly located in the nucleus, where Rad23B associates with the proteasome through its N-terminus, facilitating the degradation of damaged proteins and thus maintaining cellular homeostasis. The C-terminal ubiquitin-associated domain of Rad23B protein is highly conserved across species, underscoring its essential function in DNA repair mechanisms. Notably, Rad23B expression is significantly higher than that of its homolog Rad23A in typical mammalian cells, which highlights Rad23B importance in the cellular response to DNA damage. Furthermore, RAD23 genes, which encode Rad23 proteins, exhibit increased transcription in response to DNA damage, making Rad23B protein a crucial player in the cellular defense against genotoxic stress.

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Rad23B Antibody (H-8) References:

1. Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination.  |  Elder, RT., et al. 2002. Nucleic Acids Res. 30: 581-91. PMID: 11788722
2. A novel regulation mechanism of DNA repair by damage-induced and RAD23-dependent stabilization of xeroderma pigmentosum group C protein.  |  Ng, JM., et al. 2003. Genes Dev. 17: 1630-45. PMID: 12815074
3. Ubiquitin recognition by the DNA repair protein hHR23a.  |  Wang, Q., et al. 2003. Biochemistry. 42: 13529-35. PMID: 14621999
4. Structure of the XPC binding domain of hHR23A reveals hydrophobic patches for protein interaction.  |  Kamionka, M. and Feigon, J. 2004. Protein Sci. 13: 2370-7. PMID: 15322280
5. Relative levels of the two mammalian Rad23 homologs determine composition and stability of the xeroderma pigmentosum group C protein complex.  |  Okuda, Y., et al. 2004. DNA Repair (Amst). 3: 1285-95. PMID: 15336624
6. The UBA2 domain functions as an intrinsic stabilization signal that protects Rad23 from proteasomal degradation.  |  Heessen, S., et al. 2005. Mol Cell. 18: 225-35. PMID: 15837425
7. Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B.  |  Kim, B., et al. 2005. FEBS J. 272: 2467-76. PMID: 15885096
8. HHR23A, a human homolog of Saccharomyces cerevisiae Rad23, regulates xeroderma pigmentosum C protein and is required for nucleotide excision repair.  |  Hsieh, HC., et al. 2005. Biochem Biophys Res Commun. 335: 181-7. PMID: 16105547
9. Evidence for distinct functions for human DNA repair factors hHR23A and hHR23B.  |  Chen, L. and Madura, K. 2006. FEBS Lett. 580: 3401-8. PMID: 16712842
10. Molecular basis for damage recognition and verification by XPC-RAD23B and TFIIH in nucleotide excision repair.  |  Mu, H., et al. 2018. DNA Repair (Amst). 71: 33-42. PMID: 30174301
11. Cytoplasmic RAD23B interacts with CORO1C to synergistically promote colorectal cancer progression and metastasis.  |  Li, J., et al. 2021. Cancer Lett. 516: 13-27. PMID: 34062216
12. Knockdown of circ-RAD23B inhibits non-small cell lung cancer progression via the miR-142-3p/MAP4K3 axis.  |  Zhuang, Q., et al. 2022. Thorac Cancer. 13: 750-760. PMID: 35106926

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad23B Antibody (H-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad23B Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad23B Antibody (H-8) Specifications

Alternate Product Name: Rad23B

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 77-196

Epitope Target: Rad23B

Epitope Species: mouse

Product Reactivity/Detection: Rad23B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Rad23B Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin