Rad21 Lentiviral Activation Particles (h): sc-402348-LAC

Human RAD21 encodes Rad21, a core kleisin subunit of the cohesin complex that tethers sister chromatids and organizes higher-order chromatin architecture. Rad21 is required for accurate chromosome segregation, DNA double-strand break repair, and regulation of gene expression through enhancer–promoter communication and topologically associating domain boundaries. Through cohesin’s coordination with CTCF and replication/repair factors, RAD21 influences cell-cycle progression and genome stability pathways. Perturbation of RAD21 dosage or cohesin function is linked to cohesinopathies and has been associated with altered transcriptional programs and chromosomal instability in cancer biology research.

Rad21 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient RAD21 upregulation across a broader range of human cell types.

Rad21 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the RAD21 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Rad21 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native RAD21 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.