Date published: 2025-10-21

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Rad1 Antibody (G-6): sc-166495

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Datasheets
  • Rad1 Antibody (G-6) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 1-282 representing full length Rad1 of human origin
  • Rad1 Antibody (G-6) is recommended for detection of Rad1 of human origin by WB, IP, IF and ELISA
  • Anti-Rad1 Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rad1 Antibody (G-6) for WB applications. These reagents are now offered in bundles with Rad1 Antibody (G-6) (see ordering information below).

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    Rad1 Antibody (G-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects human Rad1 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad1 Antibody (G-6) comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. Rad1 (G-6) antibody recognizes a protein crucial for DNA damage response and cell cycle checkpoint regulation, maintaining genomic stability. Rad1 functions as part of the 9-1-1 complex, essential for activating Chk1 kinase during DNA damage response, enabling cell cycle arrest and DNA repair. This mechanism prevents damaged DNA propagation that could lead to tumorigenesis. Rad1 interactions with Rad9 and Hus1 emphasize DNA damage response pathway importance, working together for proper cell cycle regulation and repair. Rad1 phosphorylation during DNA damage highlights cellular signaling pathway involvement in determining cell fate following genomic stress.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Rad1 Antibody (G-6)

    sc-166495
    200 µg/ml
    $316.00

    Rad1 Antibody (G-6): m-IgG Fc BP-HRP Bundle

    sc-527165
    200 µg Ab; 10 µg BP
    $354.00

    Rad1 Antibody (G-6): m-IgG1 BP-HRP Bundle

    sc-532538
    200 µg Ab; 20 µg BP
    $354.00

    Rad1 Antibody (G-6) AC

    sc-166495 AC
    500 µg/ml, 25% agarose
    $416.00

    Rad1 Antibody (G-6) HRP

    sc-166495 HRP
    200 µg/ml
    $316.00

    Rad1 Antibody (G-6) FITC

    sc-166495 FITC
    200 µg/ml
    $330.00

    Rad1 Antibody (G-6) PE

    sc-166495 PE
    200 µg/ml
    $343.00

    Rad1 Antibody (G-6) Alexa Fluor® 488

    sc-166495 AF488
    200 µg/ml
    $357.00

    Rad1 Antibody (G-6) Alexa Fluor® 546

    sc-166495 AF546
    200 µg/ml
    $357.00

    Rad1 Antibody (G-6) Alexa Fluor® 594

    sc-166495 AF594
    200 µg/ml
    $357.00

    Rad1 Antibody (G-6) Alexa Fluor® 647

    sc-166495 AF647
    200 µg/ml
    $357.00

    Rad1 Antibody (G-6) Alexa Fluor® 680

    sc-166495 AF680
    200 µg/ml
    $357.00

    Rad1 Antibody (G-6) Alexa Fluor® 790

    sc-166495 AF790
    200 µg/ml
    $357.00

    What blocking solution should I use with Rad1 (G-6): sc-166495 monoclonal antibody for Western blot?

    Asked by: Cweed
    Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-02-28
    • y_2025, m_10, d_14, h_6CST
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    Rated 3 out of 5 by from Clean band at expected heightI have tested the antibody for the Turboblot/Odyssey system. I used the Rad1 antibody 1:100 in 5% BSA, and used Goat anti-mouse Odyssey compatible secondary antibody. A clean band appeared around 29 kDa (although signal was relatively low).
    Date published: 2018-01-02
    Rated 5 out of 5 by from Works great for us!Got good, strong bands in U2OS cell lysates. Used the antibody at 1:500 dilution overnight.
    Date published: 2017-07-27
    Rated 5 out of 5 by from Strong band in WB in human Rad1 transfectedStrong band in WB in human Rad1 transfected 293T whole cell lysate. -SCBT QC
    Date published: 2014-10-02
    • y_2025, m_10, d_14, h_6
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    Rad1 Antibody (G-6) is rated 4.3 out of 5 by 3.
    • y_2025, m_10, d_14, h_6
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