Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RACK1 Double Nickase Plasmid (m): sc-420608-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RACK1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RACK1 Double Nickase Plasmid (m) and RACK1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Rack1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RACK1 Antibody (B-3): sc-17754
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RACK1 Double Nickase Plasmid (m) | sc-420608-NIC | 20 µg | $410.00 |
Rack1 encodes receptor for activated C kinase 1 (RACK1), a conserved WD40 repeat scaffolding protein that organizes multiprotein signaling complexes and links receptor inputs to downstream kinase pathways. In mouse cells, RACK1 associates with PKC isoforms and integrates signaling through MAPK/ERK, Src-family kinases, and PI3K-related networks while also functioning on the 40S ribosomal subunit to modulate translation initiation and stress-responsive protein synthesis. Through these roles, RACK1 influences cell adhesion, polarity, migration, and cell-cycle progression via coordination of focal adhesion and cytoskeletal remodeling pathways. Dysregulated RACK1-dependent signaling and translational control has been associated with oncogenic signaling states, inflammatory signaling circuits, and altered responses to cellular stress, making it a frequent target for mechanistic studies.
RACK1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Rack1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Rack1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Rack1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Rack1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.