Rab11-FIP3 Double Nickase Plasmid (h): sc-405735-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab11-FIP3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab11-FIP3 Double Nickase Plasmid (h) and Rab11-FIP3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAB11FIP3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab11-FIP3 Antibody (6H6): sc-517043
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab11-FIP3 Double Nickase Plasmid (h) | sc-405735-NIC | 20 µg | $410.00 | |||
Rab11-FIP3 Double Nickase Plasmid (h2) | sc-405735-NIC-2 | 20 µg | $410.00 |
RAB11FIP3 encodes Rab11-FIP3, a Rab11 effector that couples Rab11-positive recycling endosomes to cytoskeletal and membrane remodeling machinery. It coordinates endocytic recycling, vesicle tethering, and abscission during cytokinesis, supporting proper midbody organization and completion of cell division. Through these roles it contributes to spatial control of membrane trafficking that influences receptor turnover and signaling dynamics. Dysregulation of Rab11-FIP3–linked trafficking and cytokinetic processes has been associated with proliferative and migratory phenotypes relevant to cancer cell biology and other disorders involving aberrant membrane transport.
Rab11-FIP3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAB11FIP3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAB11FIP3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAB11FIP3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAB11FIP3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.