Rab11-FIP3 CRISPR/Cas9 KO Plasmid (h): sc-405735
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab11-FIP3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab11-FIP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab11-FIP3 Antibody (6H6): sc-517043
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab11-FIP3 CRISPR/Cas9 KO Plasmid (h) | sc-405735 | 20 µg | $397.00 |
Overview
RAB11FIP3 encodes Rab11 family-interacting protein 3 (Rab11-FIP3), a Rab11 effector that coordinates recycling endosome function and membrane trafficking during cytokinesis. Rab11-FIP3 helps direct endocytic recycling and vesicle targeting to the midbody, supporting abscission and proper cell-cycle progression, and it interfaces with RAB11-dependent transport routes that shape receptor recycling and polarity. Through its role in recycling endosome dynamics, Rab11-FIP3 contributes to processes that influence cell migration and proliferation, making it relevant to studies of dysregulated trafficking in cancer-associated phenotypes. Altered expression or functional perturbation of Rab11-FIP3 has also been linked to defects in cell division and membrane remodeling pathways that can impact genomic stability.
Rab11-FIP3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAB11FIP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAB11FIP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAB11FIP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab11-FIP3 protein expression.
This CRISPR knockout system enables efficient generation of RAB11FIP3-deficient cell models for investigation of Rab11-FIP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAB11FIP3 exon(s) critical for Rab11-FIP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAB11FIP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab11-FIP3 CRISPR/Cas9 KO Plasmid (h) and Rab11-FIP3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAB11FIP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab11-FIP3 HDR Plasmid (h) and Rab11-FIP3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAB11FIP3 homology arms to support homology-directed repair at defined RAB11FIP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.