Rab11-FIP1 Double Nickase Plasmid (h): sc-407521-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab11-FIP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab11-FIP1 Double Nickase Plasmid (h) and Rab11-FIP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAB11FIP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab11-FIP1 Antibody (3A12H9D2): sc-517228
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab11-FIP1 Double Nickase Plasmid (h) | sc-407521-NIC | 20 µg | $410.00 | |||
Rab11-FIP1 Double Nickase Plasmid (h2) | sc-407521-NIC-2 | 20 µg | $410.00 |
RAB11FIP1 encodes Rab11-FIP1, a Rab11 family effector that coordinates Rab11-dependent recycling endosome trafficking and membrane recycling to the plasma membrane. Through interactions with small GTPases and motor/adaptor proteins, Rab11-FIP1 helps regulate receptor recycling, polarized transport, cytokinesis, and maintenance of epithelial cell architecture. These processes impact signaling outputs and cell motility by controlling the localization and turnover of membrane proteins, including receptors and adhesion molecules. Dysregulated endosomal recycling and Rab11-FIP1–associated trafficking programs have been linked to altered proliferative and migratory behavior in cancer biology and to broader defects in vesicle transport pathways relevant to neurobiology and metabolic regulation.
Rab11-FIP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAB11FIP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAB11FIP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAB11FIP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAB11FIP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.