Rab GDI β Double Nickase Plasmid (h): sc-404136-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab GDI β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab GDI β Double Nickase Plasmid (h) and Rab GDI β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GDI2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab GDI β Antibody (D-2): sc-515143
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab GDI β Double Nickase Plasmid (h) | sc-404136-NIC | 20 µg | $410.00 | |||
Rab GDI β Double Nickase Plasmid (h2) | sc-404136-NIC-2 | 20 µg | $410.00 |
GDI2 encodes Rab GDP dissociation inhibitor beta (Rab GDI β), a cytosolic chaperone that binds prenylated Rab GTPases and regulates their extraction from membranes and recycling between endomembrane compartments. By controlling Rab availability and membrane targeting, Rab GDI β influences vesicle budding, transport, and fusion events central to endocytosis, exocytosis, and Golgi-to-endosome trafficking. This regulation impacts receptor turnover, secretory pathway dynamics, and organelle identity within the Rab GTPase cycle. Dysregulated Rab trafficking and Rab regulator function are implicated in cellular stress responses and altered proteostasis observed across multiple disease-relevant contexts, supporting use of GDI2 perturbation to interrogate trafficking-dependent phenotypes.
Rab GDI β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GDI2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GDI2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GDI2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GDI2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.