Rab 9A Double Nickase Plasmid (h): sc-416915-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 9A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab 9A Double Nickase Plasmid (h) and Rab 9A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAB9A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 9A Antibody (Mab9): sc-53145
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 9A Double Nickase Plasmid (h) | sc-416915-NIC | 20 µg | $410.00 | |||
Rab 9A Double Nickase Plasmid (h2) | sc-416915-NIC-2 | 20 µg | $410.00 |
RAB9A encodes the small GTPase Rab 9A, a key regulator of late endosome trafficking that supports cargo sorting and transport between endosomes and the trans-Golgi network. By cycling between GDP- and GTP-bound states, Rab 9A coordinates vesicle budding, motility, and tethering events important for receptor recycling, lysosome-related pathways, and membrane homeostasis. Altered Rab9A activity has been linked to dysregulated endolysosomal transport and autophagy-adjacent processes, which are frequently implicated in neurodegenerative and infectious disease biology and in cancer-associated remodeling of intracellular trafficking. These functions make RAB9A a useful target for mechanistic studies of vesicular transport, organelle communication, and proteostasis.
Rab 9A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAB9A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAB9A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAB9A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAB9A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.