Date published: 2025-10-19

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Rab 9A Antibody (Mab9): sc-53145

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Datasheets
  • Rab 9A Antibody (Mab9) is a mouse monoclonal IgG1 κ Rab 9A antibody, cited in 2 publications, provided at 200 µg/ml
  • raised against recombinant full length canine Rab 9
  • Rab 9A Antibody (Mab9) is recommended for detection of prenylated and non-prenylated Rab 9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine, feline and canine; non cross-reactive with other Rab family members
  • Anti-Rab 9A Antibody (Mab9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rab 9A Antibody (Mab9) for WB and IHC(P) applications. These reagents are now offered in bundles with Rab 9A Antibody (Mab9) (see ordering information below).

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Rab 9A Antibody (Mab9) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Rab 9A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Rab 9A antibody (Mab9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab 9A, consisting of 201 amino acids, plays a crucial role in intracellular transport, specifically in the movement of proteins between endosomes and the trans Golgi network. This transport is vital for maintaining cellular function, as proteins are correctly sorted and delivered to their destinations, which is essential for processes such as secretion and membrane recycling. Rab 9A is localized to the cytoplasmic side of the cell membrane, the Golgi apparatus, and the endoplasmic reticulum, highlighting its importance in both exocytosis and endocytosis pathways. Proper functioning of Rab proteins, including Rab 9A, is critical for the overall efficiency of cellular transport mechanisms, making these proteins significant in various physiological and pathological processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Rab 9A Antibody (Mab9) References:

  1. cDNA cloning of a new member of the Ras superfamily, RAB9-like, on the human chromosome Xq22.1-q22.3 region.  |  Seki, N., et al. 2000. J Hum Genet. 45: 318-22. PMID: 11043518
  2. Role of Rab9 GTPase in facilitating receptor recruitment by TIP47.  |  Carroll, KS., et al. 2001. Science. 292: 1373-6. PMID: 11359012
  3. Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells.  |  Barbero, P., et al. 2002. J Cell Biol. 156: 511-8. PMID: 11827983
  4. Telomerase immortalization upregulates Rab9 expression and restores LDL cholesterol egress from Niemann-Pick C1 late endosomes.  |  Walter, M., et al. 2003. J Lipid Res. 44: 243-53. PMID: 12576506
  5. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability.  |  Ganley, IG., et al. 2004. Mol Biol Cell. 15: 5420-30. PMID: 15456905
  6. TIP47 is a key effector for Rab9 localization.  |  Aivazian, D., et al. 2006. J Cell Biol. 173: 917-26. PMID: 16769818
  7. Rab9A is required for delivery of cargo from recycling endosomes to melanosomes.  |  Mahanty, S., et al. 2016. Pigment Cell Melanoma Res. 29: 43-59. PMID: 26527546
  8. Circ_0013359 facilitates the tumorigenicity of melanoma by regulating miR-136-5p/RAB9A axis.  |  Zhang, Q., et al. 2021. Open Life Sci. 16: 482-494. PMID: 34056112
  9. Noncanonical Rab9a action supports retromer-mediated endosomal exit of human papillomavirus during virus entry.  |  Choi, J. and DiMaio, D. 2023. PLoS Pathog. 19: e1011648. PMID: 37703297
  10. Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene.  |  Davies, JP., et al. 1997. Genomics. 41: 131-4. PMID: 9126495
  11. A novel Rab9 effector required for endosome-to-TGN transport.  |  Díaz, E., et al. 1997. J Cell Biol. 138: 283-90. PMID: 9230071
  12. Small GTP-binding proteins in human endothelial cells.  |  de Leeuw, HP., et al. 1998. Br J Haematol. 103: 15-9. PMID: 9792283

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rab 9A Antibody (Mab9)

sc-53145
200 µg/ml
$316.00

Rab 9A Antibody (Mab9): m-IgG Fc BP-HRP Bundle

sc-526773
200 µg Ab; 10 µg BP
$354.00

Rab 9A Antibody (Mab9): m-IgG1 BP-HRP Bundle

sc-532146
200 µg Ab; 20 µg BP
$354.00

Rab 9A Antibody (Mab9) AC

sc-53145 AC
500 µg/ml, 25% agarose
$416.00

Rab 9A Antibody (Mab9) HRP

sc-53145 HRP
200 µg/ml
$316.00

Rab 9A Antibody (Mab9) FITC

sc-53145 FITC
200 µg/ml
$330.00

Rab 9A Antibody (Mab9) PE

sc-53145 PE
200 µg/ml
$343.00

Rab 9A Antibody (Mab9) Alexa Fluor® 488

sc-53145 AF488
200 µg/ml
$357.00

Rab 9A Antibody (Mab9) Alexa Fluor® 546

sc-53145 AF546
200 µg/ml
$357.00

Rab 9A Antibody (Mab9) Alexa Fluor® 594

sc-53145 AF594
200 µg/ml
$357.00

Rab 9A Antibody (Mab9) Alexa Fluor® 647

sc-53145 AF647
200 µg/ml
$357.00

Rab 9A Antibody (Mab9) Alexa Fluor® 680

sc-53145 AF680
200 µg/ml
$357.00

Rab 9A Antibody (Mab9) Alexa Fluor® 790

sc-53145 AF790
200 µg/ml
$357.00

Can Rab 9A (Mab9): sc-53145 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, Rab 9A (Mab9): sc-53145 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 3 out of 5 by from Works in IFWe received a sample and used it in human colon carcinoma cells. We saw cytoplasmic dots in IF and a weak band in western blot.
Date published: 2017-10-25
Rated 5 out of 5 by from Cytoplasmic staining of glandular cells observedCytoplasmic staining of glandular cells observed by immunoperoxidase staining of paraffin embedded human parathyroid gland tissue. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Strong bands observed by Western Blot usingStrong bands observed by Western Blot using HeLa and K-562 cells. -SCBT QC
Date published: 2013-06-25
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Rab 9A Antibody (Mab9) is rated 4.3 out of 5 by 3.
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