Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab 7L1 CRISPR/Cas9 KO Plasmid (h): sc-403366
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 7L1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab 7L1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 7L1 Antibody (D-8): sc-398274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 7L1 CRISPR/Cas9 KO Plasmid (h) | sc-403366 | 20 µg | $397.00 |
Overview
RAB29 encodes Rab 7L1, a small Rab GTPase that regulates membrane trafficking with prominent roles at the trans-Golgi network and endosomal interfaces. Rab 7L1 participates in vesicle budding, cargo sorting, and recycling pathways that shape lysosome-related homeostasis and organelle positioning through coordinated GTP-dependent interactions with effector proteins. In human cells, RAB29 has been linked to processes relevant to neuronal and immune cell biology, including pathways involving LRRK2-associated vesicular dynamics and stress-responsive trafficking. Perturbation of Rab 7L1 function is therefore studied in the context of altered endolysosomal transport, protein turnover, and signaling outputs implicated in neurodegeneration-related mechanisms.
Rab 7L1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAB29 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAB29 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAB29 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab 7L1 protein expression.
This CRISPR knockout system enables efficient generation of RAB29-deficient cell models for investigation of Rab 7L1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAB29 exon(s) critical for Rab 7L1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAB29 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab 7L1 CRISPR/Cas9 KO Plasmid (h) and Rab 7L1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAB29 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab 7L1 HDR Plasmid (h) and Rab 7L1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAB29 homology arms to support homology-directed repair at defined RAB29 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.