Rab 5A Antibody (E-11): sc-166600

Rab 5A Antibody (E-11) is a mouse monoclonal IgG3 kappa light chain antibody that detects Rab 5A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Rab 5A antibody (E-11) is available as the non-conjugated form. Rab 5A protein plays a crucial role in the early stages of endocytosis and is essential for vesicle transport within cells. Specifically, Rab 5A localizes to early endosomes, where Rab 5A regulates vesicle fusion with target membranes, facilitating sorting and delivery of internalized materials. This localization maintains cellular homeostasis and ensures proper processing and distribution of nutrients and signaling molecules. Rab 5A interacts with various effector proteins that mediate these processes, underscoring Rab 5A′s importance in cellular trafficking pathways. Rab 5A′s ability to coordinate vesicle movement is critical for numerous physiological functions, including receptor recycling and signaling pathway regulation, making Rab 5A a key player in cellular communication and metabolism.

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Rab 5A Antibody (E-11) References:

1. The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach.  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
2. Cloning of a Rab3 isotype predominantly expressed in adipocytes.  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
3. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.  |  Zahraoui, A., et al. 1989. J Biol Chem. 264: 12394-401. PMID: 2501306
4. Molecular mechanism of Ras-related protein Rab-5A and effect of mutations in the catalytically active phosphate-binding loop.  |  Khan, FI., et al. 2017. J Biomol Struct Dyn. 35: 105-118. PMID: 26727234
5. Analysis of Gtpases Rab 5 and Rab 7 expression from macrophages infected with biofilm-producing and non biofilm-producing strains of Corynebacterium pseudotuberculosis.  |  da Conceição Aquino de Sá, M., et al. 2022. Braz J Microbiol. 53: 447-453. PMID: 35023082
6. CD2AP is Co-Expressed with Tropomyosin-Related Kinase A and Ras-Related Protein Rab-5A in Cholinergic Neurons of the Murine Basal Forebrain.  |  Fitzsimons, LA., et al. 2024. bioRxiv.. PMID: 39211110
7. Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag.  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
8. Coatomers and SNAREs in promoting membrane traffic.  |  Takizawa, PA. and Malhotra, V. 1993. Cell. 75: 593-6. PMID: 8242734
9. Friends and family: the role of the Rab GTPases in vesicular traffic.  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
10. The role of GTP-binding proteins in transport along the exocytic pathway.  |  Ferro-Novick, S. and Novick, P. 1993. Annu Rev Cell Biol. 9: 575-99. PMID: 8280472
11. Association of the low molecular weight GTP-binding protein rap2B with the cytoskeleton during platelet aggregation.  |  Torti, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 7553-7. PMID: 8356055
12. Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins.  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rab 5A Antibody (E-11) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Rab 5A Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rab 5A Antibody (E-11) Specifications

Alternate Product Name: RAB5A

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 177-211

Epitope Target: Rab 5A

Epitope Species: human

Product Reactivity/Detection: Rab 5A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Rab 5A Antibody (E-11): P: Blocking Peptide

The preferred Rab 5 antibody is Rab 5 Antibody (D-11): sc-46692.