Rab 27b Double Nickase Plasmid (m2): sc-429802-NIC-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 27b Double Nickase Plasmid (m2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab 27b Double Nickase Plasmid (m2) and Rab 27b Double Nickase Plasmid (m22) encode distinct paired gRNA designs targeting Rab27b. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 27b Antibody (1596CT245.254.49): sc-517602
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 27b Double Nickase Plasmid (m2) | sc-429802-NIC-2 | 20 µg | $410.00 |
Mouse Rab27b encodes the small GTPase Rab 27b, a Ras superfamily member that regulates vesicle trafficking by controlling docking and exocytosis of secretory granules and endosome-related organelles through interactions with effectors such as Slp/Slac2 family proteins and myosin motors. Rab27b activity contributes to regulated secretion, lysosome-related organelle dynamics, and cytoskeletal coordination, supporting processes including platelet dense granule release, neutrophil degranulation, and epithelial secretory function. Dysregulated Rab27b-dependent trafficking has been linked to altered inflammatory responses and metastatic behavior in cancer models, making this target useful for studying secretion-driven microenvironment remodeling. Rab27b reagents are applied in mechanistic research using mouse primary cells and in vivo models to interrogate granule biogenesis, exosome and secretome regulation, and vesicle transport phenotypes in immune and tumor biology.
Rab 27b Double Nickase Plasmid (m2) consists of a matched pair of plasmids engineered for high-specificity editing of the Rab27b locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Rab27b. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Rab27b function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Rab27b-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.