Rab 27b Double Nickase Plasmid (h): sc-403498-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 27b Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab 27b Double Nickase Plasmid (h) and Rab 27b Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAB27B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 27b Antibody (1596CT245.254.49): sc-517602
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 27b Double Nickase Plasmid (h) | sc-403498-NIC | 20 µg | $410.00 | |||
Rab 27b Double Nickase Plasmid (h2) | sc-403498-NIC-2 | 20 µg | $410.00 |
RAB27B encodes the small GTPase Rab 27b, a key regulator of vesicle trafficking that coordinates docking and fusion of secretory granules and exosomes at the plasma membrane. Rab 27b functions through cycling between GDP- and GTP-bound states and engages effector networks implicated in cytoskeletal remodeling, membrane dynamics, and regulated exocytosis. In human cells, RAB27B-dependent trafficking influences secretion programs relevant to immune and inflammatory signaling, platelet and neutrophil granule release, and tumor–stromal communication via extracellular vesicles. Dysregulated RAB27B expression or activity has been associated with altered vesicle secretion phenotypes observed in cancer progression and metastatic behavior, supporting its use as a mechanistic node in membrane transport research.
Rab 27b Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAB27B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAB27B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAB27B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAB27B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.