Rab 24 Double Nickase Plasmid (h): sc-407266-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 24 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab 24 Double Nickase Plasmid (h) and Rab 24 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAB24. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 24 Antibody (43): sc-136049
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 24 Double Nickase Plasmid (h) | sc-407266-NIC | 20 µg | $410.00 | |||
Rab 24 Double Nickase Plasmid (h2) | sc-407266-NIC-2 | 20 µg | $410.00 |
RAB24 encodes Rab 24, a small Rab-family GTPase that regulates membrane trafficking events linked to late endosomal dynamics, lysosome-related pathways, and autophagy. Rab 24 has been implicated in autophagosome maturation and vesicle transport processes that coordinate cellular homeostasis under stress. Altered RAB24 activity has been associated with defects in proteostasis and organelle turnover, connecting its function to neurodegeneration- and myopathy-relevant cellular phenotypes. As a trafficking regulator, Rab 24 provides a tractable node for dissecting cross-talk between endosomal sorting, lysosomal function, and autophagic flux.
Rab 24 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAB24 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAB24. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAB24 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAB24-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.