Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab 18 CRISPR/Cas9 KO Plasmid (h): sc-417193
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 18 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rab 18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 18 Antibody (D-5): sc-393168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 18 CRISPR/Cas9 KO Plasmid (h) | sc-417193 | 20 µg | $397.00 |
Overview
RAB18 encodes Rab18, a small GTPase of the Rab family that regulates intracellular membrane trafficking, with prominent roles at the endoplasmic reticulum (ER) and lipid droplet interface. Rab18 coordinates vesicle transport, organelle tethering, and ER morphology through cycling between GDP- and GTP-bound states and interactions with membrane-associated effectors. Disruption of RAB18-dependent trafficking can alter lipid homeostasis, secretory pathway dynamics, and autophagy-related processes. Genetic and functional studies have linked RAB18 dysfunction to neurodevelopmental phenotypes and cellular stress responses, supporting its relevance in models of neurological disease biology and metabolism-associated pathways.
Rab 18 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAB18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAB18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAB18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rab 18 protein expression.
This CRISPR knockout system enables efficient generation of RAB18-deficient cell models for investigation of Rab 18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAB18 exon(s) critical for Rab 18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAB18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rab 18 CRISPR/Cas9 KO Plasmid (h) and Rab 18 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAB18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rab 18 HDR Plasmid (h) and Rab 18 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAB18 homology arms to support homology-directed repair at defined RAB18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.