Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab 14 Double Nickase Plasmid (m): sc-427035-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 14 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab 14 Double Nickase Plasmid (m) and Rab 14 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Rab14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 14 Antibody (D-5): sc-271401
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 14 Double Nickase Plasmid (m) | sc-427035-NIC | 20 µg | $410.00 | |||
Rab 14 Double Nickase Plasmid (m2) | sc-427035-NIC-2 | 20 µg | $410.00 |
Mouse Rab14 encodes the small GTPase Rab 14, a member of the RAS superfamily that coordinates intracellular membrane trafficking by cycling between GDP- and GTP-bound states. Rab 14 regulates endocytic recycling and biosynthetic transport between the Golgi, endosomes, and plasma membrane, influencing cargo sorting, receptor turnover, and vesicle maturation through interactions with effectors and SNARE machinery. These processes intersect with signaling, nutrient uptake, and cytoskeletal organization, making Rab14 a useful node for studying perturbations in membrane dynamics. Dysregulated Rab14-dependent trafficking has been linked in the literature to altered cell migration and metabolic homeostasis and is frequently investigated in models of proliferative and neurobiological disorders where vesicular transport contributes to phenotype.
Rab 14 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Rab14 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Rab14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Rab14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Rab14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.