QTRT1 Double Nickase Plasmid (h): sc-413456-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- QTRT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- QTRT1 Double Nickase Plasmid (h) and QTRT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting QTRT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: QTRT1 Antibody (D-7): sc-398918
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
QTRT1 Double Nickase Plasmid (h) | sc-413456-NIC | 20 µg | $410.00 |
QTRT1 encodes the catalytic subunit of tRNA-guanine transglycosylase, which installs queuosine at the wobble position (G34) of specific cytosolic tRNAs, thereby shaping codon decoding fidelity and translational dynamics. Through this tRNA modification pathway, QTRT1 contributes to proteostasis and cellular stress adaptation by influencing the accuracy and efficiency of protein synthesis. Dysregulation of tRNA modification enzymes, including QTRT1, has been associated with altered translational programs linked to proliferation and stress-response phenotypes, making the gene relevant to studies of RNA biology and metabolic regulation. QTRT1 is therefore a useful target for mechanistic interrogation of how wobble-base modifications impact gene expression at the level of translation.
QTRT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the QTRT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within QTRT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt QTRT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of QTRT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.