QKI Antibody (N147/6): sc-517305

QKI Antibody (N147/6) is a mouse monoclonal IgG2b antibody that detects QKI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-QKI antibody (N147/6) is available as the non-conjugated format. QKI protein, also known as HKQ, QK, QK3, or quaking, is a 341 amino acid protein that is crucial for proper cellular function, being primarily located in both cytoplasm and nucleus. This dual localization allows QKI to interact with RNA in various cellular contexts, facilitating QKI′s role as an RNA-binding protein essential for myelination. QKI specifically binds to RNA core sequence 5′-NACUAAY-N(1,20)-UAAY-3′, which is vital for regulating pre-mRNA splicing and mRNA export, thereby promoting stability of select mRNAs. QKI undergoes post-translational modifications, including methylation by PRMT1 and phosphorylation at its C-terminus, which can influence mRNA-binding affinity. Defects or deletions in QKI gene have been linked to astrocytic tumors and may play a role in schizophrenia pathogenesis. Multiple isoforms of QKI arise from alternative splicing events, highlighting QKI′s complexity and regulatory potential in various biological processes.

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QKI Antibody (N147/6) References:

1. Quaking is essential for blood vessel development.  |  Noveroske, JK., et al. 2002. Genesis. 32: 218-30. PMID: 11892011
2. Function of quaking in myelination: regulation of alternative splicing.  |  Wu, JI., et al. 2002. Proc Natl Acad Sci U S A. 99: 4233-8. PMID: 11917126
3. Retrotransposon-mediated restoration of Chlorella telomeres: accumulation of Zepp retrotransposons at termini of newly formed minichromosomes.  |  Yamamoto, Y., et al. 2003. Nucleic Acids Res. 31: 4646-53. PMID: 12888526
4. Human QKI, a potential regulator of mRNA expression of human oligodendrocyte-related genes involved in schizophrenia.  |  Aberg, K., et al. 2006. Proc Natl Acad Sci U S A. 103: 7482-7. PMID: 16641098
5. Developmental expression profile of quaking, a candidate gene for schizophrenia, and its target genes in human prefrontal cortex and hippocampus shows regional specificity.  |  Lauriat, TL., et al. 2008. J Neurosci Res. 86: 785-96. PMID: 17918747
6. Mature myelin maintenance requires Qki to coactivate PPARβ-RXRα-mediated lipid metabolism.  |  Zhou, X., et al. 2020. J Clin Invest. 130: 2220-2236. PMID: 32202512
7. Qki is an essential regulator of microglial phagocytosis in demyelination.  |  Ren, J., et al. 2021. J Exp Med. 218: PMID: 33045062
8. Qki regulates myelinogenesis through Srebp2-dependent cholesterol biosynthesis.  |  Zhou, X., et al. 2021. Elife. 10: PMID: 33942715
9. QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism.  |  Zhao, Z., et al. 2023. Cell. 186: 3208-3226.e27. PMID: 37379838
10. The quaking gene product necessary in embryogenesis and myelination combines features of RNA binding and signal transduction proteins.  |  Ebersole, TA., et al. 1996. Nat Genet. 12: 260-5. PMID: 8589716
11. Neural cell type-specific expression of QKI proteins is altered in quakingviable mutant mice.  |  Hardy, RJ., et al. 1996. J Neurosci. 16: 7941-9. PMID: 8987822
12. Structure-function analysis of Qk1: a lethal point mutation in mouse quaking prevents homodimerization.  |  Chen, T. and Richard, S. 1998. Mol Cell Biol. 18: 4863-71. PMID: 9671495

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-QKI Antibody (N147/6) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-QKI Antibody (N147/6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

QKI Antibody (N147/6) Specifications

Alternate Product Name: QKI

Product Clone Name: N147/6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 1-341

Epitope Target: QKI-5

Epitope Species: human

Product Reactivity/Detection: pan QKI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide, 1% Glycerol, and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for QKI Antibody (N147/6): N/A