Qa-2 Antibody (695H1-9-9): sc-52552

Qa-2 Antibody (695H1-9-9) is a mouse monoclonal IgG2a antibody that detects Qa-2 protein of mouse origin by flow cytometry (FCM). Anti-Qa-2 antibody (695H1-9-9) is available in both non-conjugated and various conjugated forms, including fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. The Qa-2 protein, a non-classical major histocompatibility complex (MHC) class I cell surface antigen, plays a crucial role in the immune system by influencing the rate of embryonic cleavage during preimplantation stages of development and participating in both adaptive and innate immune responses. Qa-2 (695H1-9-9) antibody is involved in resistance to T. crassiceps cysticercosis, highlighting its importance in host defense mechanisms. Qa-2′s unique ability to associate with a diverse array of peptide sequences is essential for function, as Qa-2 requires two dominant C-terminal anchor residues for effective peptide binding. This characteristic allows Qa-2 to present a variety of peptides to T cells, thereby modulating immune responses. The molecular weight and subunit structure of Qa-2 molecules are similar to H2 antigens, with Qa-2 heavy chain featuring two additional NH2-terminal amino acids and several critical amino acid interchanges, which may influence binding properties and interactions with other proteins.

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Qa-2 Antibody (695H1-9-9) References:

1. Promiscuous antigen presentation by the nonclassical MHC Ib Qa-2 is enabled by a shallow, hydrophobic groove and self-stabilized peptide conformation.  |  He, X., et al. 2001. Structure. 9: 1213-24. PMID: 11738047
2. Correction of defects responsible for impaired Qa-2 class Ib MHC expression on melanoma cells protects mice from tumor growth.  |  Chiang, EY., et al. 2003. J Immunol. 170: 4515-23. PMID: 12707328
3. Evidence that HLA-G is the functional homolog of mouse Qa-2, the Ped gene product.  |  Comiskey, M., et al. 2003. Hum Immunol. 64: 999-1004. PMID: 14602227
4. In vivo effects of monoclonal antibodies to distinct epitopes of Qa-2 antigens.  |  Sharabi, Y. and Sachs, DH. 1990. J Exp Med. 171: 211-9. PMID: 1688608
5. HLA-G is found in lipid rafts and can act as a signaling molecule.  |  Comiskey, M., et al. 2007. Hum Immunol. 68: 1-11. PMID: 17207707
6. Epitope clusters of Qa-2 antigens defined by a panel of new monoclonal antibodies.  |  Sharrow, SO., et al. 1989. J Immunol. 142: 3495-502. PMID: 2469717
7. Primary structural studies of the Qa-2 alloantigen: implications for the evolution of the MHC.  |  Soloski, MJ., et al. 1982. Nature. 296: 759-61. PMID: 7070515
8. Biochemical analysis of an MHC-linked hematopoietic cell surface antigen, Qa-2.  |  Soloski, MJ., et al. 1981. J Supramol Struct Cell Biochem. 16: 167-77. PMID: 7299842
9. Increased resistance to Taenia crassiceps murine cysticercosis in Qa-2 transgenic mice.  |  Fragoso, G., et al. 1998. Infect Immun. 66: 760-4. PMID: 9453638