Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
pyridoxal phosphatase CRISPR/Cas9 KO Plasmid (h): sc-406810
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- pyridoxal phosphatase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the pyridoxal phosphatase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: pyridoxal phosphatase Antibody (F-2): sc-271379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
pyridoxal phosphatase CRISPR/Cas9 KO Plasmid (h) | sc-406810 | 20 µg | $397.00 |
Overview
PDXP encodes pyridoxal phosphatase, a cytosolic enzyme that hydrolyzes pyridoxal 5′-phosphate (PLP), the active form of vitamin B6, thereby contributing to cellular control of PLP availability and B6 vitamer homeostasis. Because PLP is an essential cofactor for numerous aminotransferases, decarboxylases, and enzymes in one-carbon and neurotransmitter-related metabolism, PDXP activity can indirectly influence amino acid turnover and broader metabolic flux. Through its role in maintaining cofactor balance, PDXP is relevant to studies of metabolic adaptation, redox-linked pathways, and enzyme-cofactor regulation. Altered PLP-dependent metabolism has been implicated in diverse disease-relevant contexts, making PDXP a useful target for mechanistic investigations of metabolism-associated cellular phenotypes.
pyridoxal phosphatase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PDXP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PDXP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PDXP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish pyridoxal phosphatase protein expression.
This CRISPR knockout system enables efficient generation of PDXP-deficient cell models for investigation of pyridoxal phosphatase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PDXP exon(s) critical for pyridoxal phosphatase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PDXP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by pyridoxal phosphatase CRISPR/Cas9 KO Plasmid (h) and pyridoxal phosphatase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PDXP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by pyridoxal phosphatase HDR Plasmid (h) and pyridoxal phosphatase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PDXP homology arms to support homology-directed repair at defined PDXP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.