PXDN Double Nickase Plasmid (h): sc-404707-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PXDN Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PXDN Double Nickase Plasmid (h) and PXDN Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PXDN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PXDN Antibody (2C11): sc-293408
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PXDN Double Nickase Plasmid (h) | sc-404707-NIC | 20 µg | $410.00 | |||
PXDN Double Nickase Plasmid (h2) | sc-404707-NIC-2 | 20 µg | $410.00 |
PXDN (peroxidasin) encodes a heme peroxidase that localizes to the extracellular matrix and catalyzes sulfilimine crosslink formation in collagen IV, supporting basement membrane assembly and tissue integrity. Through peroxide-dependent chemistry and halide utilization, PXDN contributes to redox homeostasis and extracellular matrix remodeling, influencing cell adhesion, migration, and developmental morphogenesis. Altered PXDN activity or expression has been associated with defects in basement membrane architecture and ocular/vascular phenotypes, making it relevant to studies of fibrosis, inflammation-associated remodeling, and oxidative stress responses. PXDN is therefore widely investigated in pathways linking extracellular matrix stability with redox signaling and epithelial–mesenchymal interactions.
PXDN Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PXDN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PXDN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PXDN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PXDN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.