PUS1 Antibody (E-3): sc-376332

PUS1 Antibody (E-3) is a mouse monoclonal IgG1 antibody that detects PUS1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PUS1, or Pseudouridine synthase 1, plays a crucial role in the post-transcriptional modification of RNA, specifically in the conversion of uridine to pseudouridine, which is essential for the stability and function of tRNA and rRNA molecules. This modification enhances the structural integrity of RNA, thereby influencing protein synthesis and cellular function. PUS1 is predominantly located in the nucleus, where PUS1 forms complexes with other proteins, such as RARG and SRA1 RNA, facilitating its role in the peptidyl transfer reaction during protein synthesis. PUS1 expression is notably high in brain and skeletal muscle tissues, reflecting its importance in these areas, particularly given that defects in PUS1 are linked to myopathy with lactic acidosis and sideroblastic anemia (MLASA), a rare genetic disorder affecting oxidative phosphorylation in muscle and bone marrow. The human PUS1 protein consists of 348 amino acids and exhibits a remarkable 92% sequence homology with its mouse counterpart, underscoring its evolutionary conservation and functional significance across species. Anti-PUS1 antibody (E-3) is an invaluable tool for researchers investigating the molecular mechanisms underlying RNA modifications and their implications in various diseases.

The preferred PUS1 antibody is PUS1 Antibody (C-4): sc-376442.