Purβ Antibody (211H2H): sc-517644

Purβ Antibody (211H2H) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Pur beta protein of mouse, rat, and human origin by western blotting (WB). Anti-Purβ antibody (211H2H) is available as the non-conjugated form. Purβ, or Purine-rich element-binding protein B, is a 312 amino acid protein that plays a crucial role in gene regulation and DNA replication. Purβ belongs to the PUR DNA-binding protein family and functions as a sequence-specific, single-stranded DNA-binding protein, preferentially binding to purine-rich elements found at origins of replication and in gene flanking regions across various eukaryotic species, from yeasts to humans. This binding is essential for proper control of transcription and DNA replication, highlighting Purβ′s importance in cellular processes. Notably, Purβ has been implicated in hematological malignancies, as its deletion is associated with myelodysplastic syndrome and acute myelogenous leukemia, conditions characterized by the arrest of hematopoietic precursors in early developmental stages. Additionally, Purβ is localized in the nucleus and is expressed in the myocardium of patients with heart failure, indicating Purβ′s potential role in cardiac health. Purβ is conserved across multiple species, including mouse, rat, zebrafish, fruit fly, mosquito, Caenorhabditis elegans, Arabidopsis thaliana, and rice, and maps to human chromosome 7p13, underscoring Purβ′s evolutionary significance.

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Purβ Antibody (211H2H) References:

1. Deletions of PURA, at 5q31, and PURB, at 7p13, in myelodysplastic syndrome and progression to acute myelogenous leukemia.  |  Lezon-Geyda, K., et al. 2001. Leukemia. 15: 954-62. PMID: 11417483
2. The DNA sequence of human chromosome 7.  |  Hillier, LW., et al. 2003. Nature. 424: 157-64. PMID: 12853948
3. Single-stranded DNA-binding proteins PURalpha and PURbeta bind to a purine-rich negative regulatory element of the alpha-myosin heavy chain gene and control transcriptional and translational regulation of the gene expression. Implications in the repression of alpha-myosin heavy chain during heart failure.  |  Gupta, M., et al. 2003. J Biol Chem. 278: 44935-48. PMID: 12933792
4. Sequence of cDNA comprising the human pur gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein.  |  Bergemann, AD., et al. 1992. Mol Cell Biol. 12: 5673-82. PMID: 1448097
5. Repression of transcriptional enhancer factor-1 and activator protein-1-dependent enhancer activity by vascular actin single-stranded DNA binding factor 2.  |  Kelm, RJ., et al. 1996. J Biol Chem. 271: 24278-85. PMID: 8798674
6. Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Puralpha and Purbeta.  |  Kelm, RJ., et al. 1997. J Biol Chem. 272: 26727-33. PMID: 9334258