PTPN21 CRISPR/Cas9 KO Plasmid (h): sc-407466
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PTPN21 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PTPN21 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTPN21 CRISPR/Cas9 KO Plasmid (h) | sc-407466 | 20 µg | $397.00 |
Overview
PTPN21 encodes a cytosolic protein tyrosine phosphatase that modulates phosphorylation-dependent signaling by dephosphorylating specific substrates and scaffolding proteins. Through regulation of receptor tyrosine kinase outputs and downstream pathways controlling cytoskeletal dynamics, adhesion, and vesicular trafficking, PTPN21 can influence cell motility and signal amplitude. Its activity intersects with networks governing growth factor responses and stress signaling, making it relevant to studies of cellular transformation and invasive phenotypes. Dysregulated phosphatase signaling involving PTPN21 has been implicated in contexts such as cancer-associated signaling rewiring and altered migratory behavior.
PTPN21 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PTPN21 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PTPN21 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PTPN21 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PTPN21 protein expression.
This CRISPR knockout system enables efficient generation of PTPN21-deficient cell models for investigation of PTPN21 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PTPN21 exon(s) critical for PTPN21 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PTPN21 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PTPN21 CRISPR/Cas9 KO Plasmid (h) and PTPN21 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PTPN21 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PTPN21 HDR Plasmid (h) and PTPN21 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PTPN21 homology arms to support homology-directed repair at defined PTPN21 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.