PTP-PEST Antibody (AG25): sc-65229

PTP-PEST Antibody (AG25) is a mouse monoclonal IgG1 antibody that detects PTP-PEST in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). PTP-PEST, a 780 amino acid cytosolic nonreceptor protein encoded by a gene located on chromosome 7q11.23, plays a critical role in regulating cellular signaling pathways by modulating tyrosine phosphorylation. PTP-PEST is predominantly expressed in various hemopoietic cell types, including B cells and T cells, where PTP-PEST is involved in essential processes such as cellular growth and differentiation. Notably, PTP-PEST interacts with several key signaling molecules, including Shc, paxillin, Csk, and Cas, facilitating the dephosphorylation of these proteins and thereby influencing pathways like the Ras signaling cascade. PTP-PEST′s ability to regulate c-Abl activity through dephosphorylation represents a significant mechanism of action, highlighting PTP-PEST′s importance in cellular signaling. Furthermore, PTP-PEST is crucial for cytoskeletal organization, promoting the turnover of focal adhesions necessary for cell migration and regulating the proline, serine, threonine phosphatase interacting protein (PSTPIP)-mediated cleavage furrow formation during cell division. With versatile applications and significant biological roles, anti-PTP-PEST antibody (AG25) serves as an invaluable tool for researchers studying tyrosine phosphorylation and its implications in various cellular processes.

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PTP-PEST Antibody (AG25) References:

1. PTP-PEST controls motility through regulation of Rac1.  |  Sastry, SK., et al. 2002. J Cell Sci. 115: 4305-16. PMID: 12376562
2. Protein tyrosine phosphatase-PEST (PTP-PEST) regulates mast cell-activating signals in PTP activity-dependent and -independent manners.  |  Motohashi, S., et al. 2014. Cell Immunol. 289: 128-34. PMID: 24791697
3. PTP-PEST controls EphA3 activation and ephrin-induced cytoskeletal remodelling.  |  Mansour, M., et al. 2016. J Cell Sci. 129: 277-89. PMID: 26644181
4. ACTH Modulates PTP-PEST Activity and Promotes Its Interaction With Paxillin.  |  Gorostizaga, AB., et al. 2016. J Cell Biochem. 117: 2170-81. PMID: 27061092
5. PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells.  |  Chen, Z., et al. 2018. Cancer Res. 78: 3809-3822. PMID: 29743287
6. Targeting protein tyrosine phosphatase PTP-PEST (PTPN12) for therapeutic intervention in acute myocardial infarction.  |  Yang, CF., et al. 2020. Cardiovasc Res. 116: 1032-1046. PMID: 31228185
7. The protein tyrosine phosphatase PTP-PEST mediates hypoxia-induced endothelial autophagy and angiogenesis via AMPK activation.  |  Chandel, S., et al. 2021. J Cell Sci. 134: PMID: 33323505
8. PTP-PEST Regulated Membranous/Cytoplasmic Translocation of p120ctn in the Lung Cancer Resistance to Tyrosine Kinase Inhibitor.  |  Liu, WW., et al. 2022. Appl Immunohistochem Mol Morphol. 30: 215-224. PMID: 35030104
9. Phosphorylation/dephosphorylation of PTP-PEST at Serine 39 is crucial for cell migration.  |  Honda, R., et al. 2023. J Biochem. 173: 73-84. PMID: 36250939
10. In-silico identification of Tyr232 in AMPKα2 as a dephosphorylation site for the protein tyrosine phosphatase PTP-PEST.  |  Manikandan, A., et al. 2023. Proteins. 91: 831-846. PMID: 36645312

The preferred PTP-PEST antibody is PTP-PEST Antibody (H-11): sc-271351.