PSTK Antibody (B-5): sc-373991

PSTK Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSTK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PSTK (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphoseryl-tRNA kinase (PSTK) protein plays a crucial role in the synthesis of selenocysteine, which is essential for the function of several selenoproteins that are vital for antioxidant defense and redox regulation in cells. By catalyzing the conversion of seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), PSTK ensures the proper incorporation of selenocysteine into proteins, thereby influencing various biological processes, including cellular signaling and metabolism. This enzyme, consisting of 348 amino acids, is part of the L-seryl-tRNA(Sec) kinase family and is encoded by a gene located on human chromosome 10q26.13. PSTK exists in two alternatively spliced isoforms, highlighting its functional diversity and importance in cellular physiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PSTK Antibody (B-5) References:

1. Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase.  |  Carlson, BA., et al. 2004. Proc Natl Acad Sci U S A. 101: 12848-53. PMID: 15317934
2. A novel bacterial signalling system with a combination of a Ser/Thr kinase cascade and a His/Asp two-component system.  |  Lux, R. and Shi, W. 2005. Mol Microbiol. 58: 345-8. PMID: 16194223
3. RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea.  |  Yuan, J., et al. 2006. Proc Natl Acad Sci U S A. 103: 18923-7. PMID: 17142313
4. Structural insights into RNA-dependent eukaryal and archaeal selenocysteine formation.  |  Araiso, Y., et al. 2008. Nucleic Acids Res. 36: 1187-99. PMID: 18158303
5. Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation.  |  Sherrer, RL., et al. 2008. Nucleic Acids Res. 36: 1247-59. PMID: 18174226
6. Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase.  |  Sherrer, RL., et al. 2008. Nucleic Acids Res. 36: 1871-80. PMID: 18267971
7. Down-regulation of the hepatic selenoprotein biosynthesis machinery impairs selenium metabolism during the acute phase response in mice.  |  Renko, K., et al. 2009. FASEB J. 23: 1758-65. PMID: 19136613
8. The canonical pathway for selenocysteine insertion is dispensable in Trypanosomes.  |  Aeby, E., et al. 2009. Proc Natl Acad Sci U S A. 106: 5088-92. PMID: 19279205
9. Structure of a tRNA-dependent kinase essential for selenocysteine decoding.  |  Araiso, Y., et al. 2009. Proc Natl Acad Sci U S A. 106: 16215-20. PMID: 19805283

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSTK Antibody (B-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PSTK Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSTK Antibody (B-5) Specifications

Alternate Product Name: PSTK

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: PSTK

Epitope Species: human

Product Reactivity/Detection: PSTK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PSTK Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin