PSS1 Double Nickase Plasmid (h): sc-404364-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PSS1 Double Nickase Plasmid (h) and PSS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTDSS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSS1 Antibody (B-5): sc-515376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSS1 Double Nickase Plasmid (h) | sc-404364-NIC | 20 µg | $410.00 | |||
PSS1 Double Nickase Plasmid (h2) | sc-404364-NIC-2 | 20 µg | $410.00 |
PTDSS1 encodes phosphatidylserine synthase 1 (PSS1), an endoplasmic reticulum membrane enzyme that catalyzes base-exchange reactions to generate phosphatidylserine from phosphatidylcholine. By controlling cellular phosphatidylserine abundance, PSS1 helps regulate membrane biogenesis, vesicular trafficking, mitochondrial–ER lipid exchange, and apoptotic signaling linked to phosphatidylserine externalization. PTDSS1 activity interfaces with broader phospholipid and sphingolipid metabolic networks that shape organelle identity and signal transduction. Dysregulated phosphatidylserine homeostasis has been associated with perturbations in neuronal function and membrane-related pathologies, supporting PTDSS1 as a target for mechanistic studies of lipid-driven cellular phenotypes.
PSS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTDSS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTDSS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTDSS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTDSS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.