PSS1 Antibody (B-5): sc-515376
- PSS1 Antibody (B-5) is a mouse monoclonal IgG1 κ PSS1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 409-435 near the C-terminus of PSS1 of human origin
- PSS1 Antibody (B-5) is recommended for detection of PSS1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PSS1 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for PSS1 Antibody (B-5) for WB applications. This reagent is now offered in a bundle with PSS1 Antibody (B-5) (see ordering information below).
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PSS1 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PSS1 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSS1 plays a crucial role in the biosynthesis of phosphatidylserine (PtdSer), a vital anionic phospholipid predominantly found in the inner layer of the plasma membrane. PSS1 functions as a membrane-associated enzyme that catalyzes the calcium-dependent base exchange of serine with existing phospholipid head groups, specifically utilizing phosphatidylcholine as its primary substrate. PSS1′s importance lies in its regulatory function, as PtdSer directly inhibits PSS1, serving as a feedback mechanism to control PtdSer synthesis. This regulation is critical for maintaining cellular homeostasis, particularly in response to stressors such as UV exposure, where cells overexpressing PSS1 exhibit significant resistance to apoptosis. Understanding PSS1′s function and regulation is essential for elucidating its role in cellular signaling and membrane dynamics, making anti-PSS1 antibody (B-5) a valuable tool for researchers studying phospholipid metabolism and related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PSS1 Antibody (B-5) References:
- Cloning and expression of murine liver phosphatidylserine synthase (PSS)-2: differential regulation of phospholipid metabolism by PSS1 and PSS2. | Stone, SJ. and Vance, JE. 1999. Biochem J. 342 (Pt 1): 57-64. PMID: 10432300
- Phosphatidylserine synthase-1 and -2 are localized to mitochondria-associated membranes. | Stone, SJ. and Vance, JE. 2000. J Biol Chem. 275: 34534-40. PMID: 10938271
- Metabolism and functions of phosphatidylserine in mammalian brain. | Mozzi, R., et al. 2003. Neurochem Res. 28: 195-214. PMID: 12608694
- Biosynthetic regulation and intracellular transport of phosphatidylserine in mammalian cells. | Kuge, O. and Nishijima, M. 2003. J Biochem. 133: 397-403. PMID: 12761285
- Purification and characterization of Chinese hamster phosphatidylserine synthase 2. | Kuge, O., et al. 2003. J Biol Chem. 278: 42692-8. PMID: 12912985
- Externalization of phosphatidylserine during apoptosis does not specifically require either isoform of phosphatidylserine synthase. | Grandmaison, PA., et al. 2004. Biochim Biophys Acta. 1636: 1-11. PMID: 14984733
- Resistance to UV-induced apoptosis in Chinese-hamster ovary cells overexpressing phosphatidylserine synthases. | Yu, A., et al. 2004. Biochem J. 381: 609-18. PMID: 15099192
- Functional analysis of Chinese hamster phosphatidylserine synthase 1 through systematic alanine mutagenesis. | Ohsawa, T., et al. 2004. Biochem J. 381: 853-9. PMID: 15130088
- Comparative genomics and evolution of eukaryotic phospholipid biosynthesis. | Lykidis, A. 2007. Prog Lipid Res. 46: 171-99. PMID: 17512056
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSS1 Antibody (B-5) | sc-515376 | 200 µg/ml | $316.00 | |||
PSS1 Antibody (B-5): m-IgG1 BP-HRP Bundle | sc-531807 | 200 µg Ab; 20 µg BP | $354.00 | |||
PSS1 Antibody (B-5) AC | sc-515376 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PSS1 Antibody (B-5) HRP | sc-515376 HRP | 200 µg/ml | $316.00 | |||
PSS1 Antibody (B-5) FITC | sc-515376 FITC | 200 µg/ml | $330.00 | |||
PSS1 Antibody (B-5) PE | sc-515376 PE | 200 µg/ml | $343.00 | |||
PSS1 Antibody (B-5) Alexa Fluor® 488 | sc-515376 AF488 | 200 µg/ml | $357.00 | |||
PSS1 Antibody (B-5) Alexa Fluor® 546 | sc-515376 AF546 | 200 µg/ml | $357.00 | |||
PSS1 Antibody (B-5) Alexa Fluor® 594 | sc-515376 AF594 | 200 µg/ml | $357.00 | |||
PSS1 Antibody (B-5) Alexa Fluor® 647 | sc-515376 AF647 | 200 µg/ml | $357.00 | |||
PSS1 Antibody (B-5) Alexa Fluor® 680 | sc-515376 AF680 | 200 µg/ml | $357.00 | |||
PSS1 Antibody (B-5) Alexa Fluor® 790 | sc-515376 AF790 | 200 µg/ml | $357.00 | |||
PSS1 (B-5) Neutralizing Peptide | sc-515376 P | 100 µg/0.5 ml | $68.00 |