PSMC3 Antibody (28-K): sc-100462

PSMC3 Antibody (28-K) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSMC3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PSMC3 antibody (28-K) is available as a non-conjugated monoclonal isotype antibody. PSMC3, also known as Proteasome 26S subunit ATPase 3 or TBP1 (Tat-binding protein 1), plays a crucial role in selective degradation of cellular proteins, a process vital for maintaining cellular homeostasis and regulating various biological functions. PSMC3 is a 439 amino acid member of the AAA ATPase family and is localized in both nucleus and cytoplasm, where PSMC3 serves as a subunit of the 19S regulatory complex of the 26S proteasome. The 26S proteasome is essential for degradation of ubiquitinated proteins, which are marked for destruction due to damage or misfolding. PSMC3 helps regulate substrate specificity of the proteasome, ensuring that only appropriate proteins are targeted for degradation. Additionally, PSMC3 interacts with HIV protein HIV-1 Tat, facilitating association of this viral protein with transcription complexes, thereby influencing viral replication and pathogenesis. This multifaceted role of PSMC3 underscores significance in both normal cellular processes and disease mechanisms, making PSMC3 (28-K) antibody a valuable tool for researchers studying proteasome function and related pathways.

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PSMC3 Antibody (28-K) References:

1. The enzymatic activity of CEM15/Apobec-3G is essential for the regulation of the infectivity of HIV-1 virion but not a sole determinant of its antiviral activity.  |  Shindo, K., et al. 2003. J Biol Chem. 278: 44412-6. PMID: 12970355
2. Human immunodeficiency virus-1 Tat protein interacts with distinct proteasomal alpha and beta subunits.  |  Apcher, GS., et al. 2003. FEBS Lett. 553: 200-4. PMID: 14550573
3. Tat-binding protein-1, a component of the 26S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau protein.  |  Corn, PG., et al. 2003. Nat Genet. 35: 229-37. PMID: 14556007
4. The Vif protein of HIV triggers degradation of the human antiretroviral DNA deaminase APOBEC3G.  |  Conticello, SG., et al. 2003. Curr Biol. 13: 2009-13. PMID: 14614829
5. Functional and physical interaction of the human ARF tumor suppressor with Tat-binding protein-1.  |  Pollice, A., et al. 2004. J Biol Chem. 279: 6345-53. PMID: 14665636
6. Post-translationally modified S12, absent in transformed breast epithelial cells, is not associated with the 26S proteasome and is induced by proteasome inhibitor.  |  Thompson, HG., et al. 2004. Int J Cancer. 111: 338-47. PMID: 15221960
7. TBP-1 protects the human oncosuppressor p14ARF from proteasomal degradation.  |  Pollice, A., et al. 2007. Oncogene. 26: 5154-62. PMID: 17334400
8. Localization of genes encoding two human one-domain members of the AAA family: PSMC5 (the thyroid hormone receptor-interacting protein, TRIP1) and PSMC3 (the Tat-binding protein, TBP1).  |  Hoyle, J., et al. 1997. Hum Genet. 99: 285-8. PMID: 9048938
9. Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases.  |  Tanahashi, N., et al. 1998. Biochem Biophys Res Commun. 243: 229-32. PMID: 9473509

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSMC3 Antibody (28-K) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PSMC3 Antibody (28-K) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSMC3 Antibody (28-K) Specifications

Alternate Product Name: PSMC3

Product Clone Name: 28-K

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 53-152

Epitope Target: PSMC3

Epitope Species: human

Product Reactivity/Detection: PSMC3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PSMC3 Antibody (28-K): N/A