PSK2 Double Nickase Plasmid (h2): sc-418247-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSK2 Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PSK2 Double Nickase Plasmid (h2) and PSK2 Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting TAOK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSK2 Antibody (22): sc-136094
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSK2 Double Nickase Plasmid (h2) | sc-418247-NIC-2 | 20 µg | $410.00 |
Human TAOK1 (TAO kinase 1), also referred to as PSK2, encodes a serine/threonine kinase that functions upstream of MAPK signaling, particularly the stress-activated p38 and JNK cascades, to coordinate cytoskeletal dynamics, centrosome organization, and microtubule-dependent processes important for mitosis and neuronal development. TAOK1 activity integrates cues from cellular stress and polarity pathways, influencing phosphorylation networks that regulate apoptosis, migration, and neurite outgrowth. Genetic and functional studies have implicated TAOK1 dysregulation in neurodevelopmental phenotypes and cancer-associated signaling rewiring, supporting its relevance to models of altered MAPK control and cytoskeleton-driven cellular behavior. Gene editing of TAOK1 enables mechanistic interrogation of kinase-dependent pathway crosstalk, mapping of phosphorylation substrates, and generation of isogenic human cell systems to study genotype–phenotype relationships in disease-relevant contexts.
PSK2 Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the TAOK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TAOK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TAOK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TAOK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.