Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PSF Double Nickase Plasmid (h): sc-402024-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PSF Double Nickase Plasmid (h) and PSF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SFPQ. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSF Antibody (D-8): sc-271796
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSF Double Nickase Plasmid (h) | sc-402024-NIC | 20 µg | $410.00 | |||
PSF Double Nickase Plasmid (h2) | sc-402024-NIC-2 | 20 µg | $410.00 |
SFPQ encodes the splicing factor proline- and glutamine-rich protein (PSF), a multifunctional nuclear RNA- and DNA-binding factor that couples transcription with pre-mRNA splicing, 3′-end processing, and RNA nuclear retention. PSF participates in assembly and regulation of paraspeckles via interactions with NEAT1 lncRNA and related DBHS family proteins, influencing gene expression programs during stress responses. Beyond RNA metabolism, PSF contributes to genome maintenance by modulating DNA double-strand break repair and replication-associated processes through protein–protein interactions at sites of damage. Dysregulation or altered localization of SFPQ has been linked to aberrant splicing and transcriptional control observed in cancer and neurodegeneration, making it a useful node for studying RNA-processing–dependent disease mechanisms in human cells.
PSF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SFPQ locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SFPQ. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SFPQ function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SFPQ-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.