PSF Antibody (G-7): sc-374502

PSF Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PSF Antibody (G-7) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSF plays a crucial role in pre-mRNA splicing, a vital process for post-transcriptional regulation of gene expression, where intron sequences are removed from precursor mRNA. This splicing process is facilitated by the spliceosome, a large ribonucleoprotein complex, and PSF is essential for spliceosome formation, particularly in early stages. PSF contains two RNA recognition motifs, a proline- and glutamine-rich amino-terminal domain, and two carboxy-terminal nuclear localization signals, which are critical for function and localization. PSF is predominantly found in nucleus speckles distinct from nucleoli, and localization is influenced by the second RNA recognition motif. PSF interacts with DNA binding domains of thyroid hormone receptors and retinoic acid receptors, functioning as a repressor by recruiting histone deacetylases to these domains. PSF is expressed in developing neurons, suggesting involvement in neuronal differentiation and maturation, and undergoes proteolytic cleavage in myeloid cells, producing a shorter fragment that may have distinct functional implications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PSF Antibody (G-7) References:

1. An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles.  |  Dye, BT. and Patton, JG. 2001. Exp Cell Res. 263: 131-44. PMID: 11161712
2. PSF is an IbeA-binding protein contributing to meningitic Escherichia coli K1 invasion of human brain microvascular endothelial cells.  |  Zou, Y., et al. 2007. Med Microbiol Immunol. 196: 135-43. PMID: 17318576
3. NPM/ALK binds and phosphorylates the RNA/DNA-binding protein PSF in anaplastic large-cell lymphoma.  |  Galietta, A., et al. 2007. Blood. 110: 2600-9. PMID: 17537995
4. PTB-associated splicing factor (PSF) functions as a repressor of STAT6-mediated Ig epsilon gene transcription by recruitment of HDAC1.  |  Dong, L., et al. 2011. J Biol Chem. 286: 3451-9. PMID: 21106524
5. PSF functions as a repressor of hypoxia-induced angiogenesis by promoting mitochondrial function.  |  Dong, L., et al. 2021. Cell Commun Signal. 19: 14. PMID: 33573690
6. Targeting Epigenetic and Posttranscriptional Gene Regulation by PSF Impairs Hormone Therapy-Refractory Cancer Growth.  |  Takayama, KI., et al. 2021. Cancer Res. 81: 3495-3508. PMID: 33975881
7. Cooperative nuclear action of RNA-binding proteins PSF and G3BP2 to sustain neuronal cell viability is decreased in aging and dementia.  |  Takayama, KI., et al. 2024. Aging Cell. 23: e14316. PMID: 39155453
8. Inhibition of PSF activity overcomes resistance to treatment in cancers harboring mutant p53.  |  Takayama, KI., et al. 2024. Mol Cancer Ther.. PMID: 39625450
9. Cloning and characterization of PSF, a novel pre-mRNA splicing factor.  |  Patton, JG., et al. 1993. Genes Dev. 7: 393-406. PMID: 8449401
10. PSF and CMF, autocrine factors that regulate gene expression during growth and early development of Dictyostelium.  |  Clarke, M. and Gomer, RH. 1995. Experientia. 51: 1124-34. PMID: 8536800

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSF Antibody (G-7) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-PSF Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSF Antibody (G-7) Specifications

Alternate Product Name: PSF

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 581-660

Epitope Target: PSF

Epitope Species: human

Product Reactivity/Detection: PSF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for PSF Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PSF antibody is PSF Antibody (39-1): sc-101137.