PSF Antibody (39-1): sc-101137

PSF Antibody (39-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects PSF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PSF antibody (39-1) is available as a non-conjugated format. PSF protein, also known as polypyrimidine tract-binding protein-associated splicing factor, plays a crucial role in pre-mRNA splicing regulation, a vital process in gene expression involving intron removal from precursor mRNA. PSF protein facilitates spliceosome formation, a large ribonucleoprotein complex, particularly in early stages. PSF protein features two RNA recognition motifs, a proline- and glutamine-rich amino terminal domain, and two carboxy-terminal nuclear localization signals, critical for function and localization. PSF protein predominantly resides in nuclear speckles distinct from nucleoli, with localization influenced by its second RNA recognition motif. PSF protein interacts with DNA binding domains of thyroid hormone receptors and retinoic acid receptors, functioning as a repressor by recruiting histone deacetylases. This interaction demonstrates PSF protein′s multifaceted role in gene regulation, particularly during neuronal differentiation and maturation in developing neurons. PSF protein undergoes proteolytic cleavage in myeloid cells, resulting in a shorter fragment with potentially distinct functional implications.

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PSF Antibody (39-1) References:

1. Identification of PSF, the polypyrimidine tract-binding protein-associated splicing factor, as a developmentally regulated neuronal protein.  |  Chanas-Sacré, G., et al. 1999. J Neurosci Res. 57: 62-73. PMID: 10397636
2. Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB.  |  Meissner, M., et al. 2000. J Cell Biochem. 76: 559-66. PMID: 10653975
3. Enhanced proteolysis of pre-mRNA splicing factors in myeloid cells.  |  Shav-Tal, Y., et al. 2000. Exp Hematol. 28: 1029-38. PMID: 11008015
4. An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles.  |  Dye, BT. and Patton, JG. 2001. Exp Cell Res. 263: 131-44. PMID: 11161712
5. PSF is a novel corepressor that mediates its effect through Sin3A and the DNA binding domain of nuclear hormone receptors.  |  Mathur, M., et al. 2001. Mol Cell Biol. 21: 2298-311. PMID: 11259580
6. Binding of the polypyrimidine tract-binding protein-associated splicing factor (PSF) to the hepatitis delta virus RNA.  |  Greco-Stewart, VS., et al. Virology. 356: 35-44. PMID: 16938326
7. Phosphorylation by SR kinases regulates the binding of PTB-associated splicing factor (PSF) to the pre-mRNA polypyrimidine tract.  |  Huang, CJ., et al. 2007. FEBS Lett. 581: 223-32. PMID: 17188683
8. Interplay between polypyrimidine tract binding protein-associated splicing factor and human myometrial progesterone receptors.  |  Tyson-Capper, AJ., et al. 2009. J Mol Endocrinol. 43: 29-41. PMID: 19339282
9. PTB-associated splicing factor (PSF) is a PPARγ-binding protein and growth regulator of colon cancer cells.  |  Tsukahara, T., et al. 2013. PLoS One. 8: e58749. PMID: 23516550
10. Interaction of HIV-1 integrase with polypyrimidine tract binding protein and associated splicing factor (PSF) and its impact on HIV-1 replication.  |  Yadav, P., et al. 2019. Retrovirology. 16: 12. PMID: 31036027
11. A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction.  |  Gozani, O., et al. 1994. EMBO J. 13: 3356-67. PMID: 8045264
12. Cloning and characterization of PSF, a novel pre-mRNA splicing factor.  |  Patton, JG., et al. 1993. Genes Dev. 7: 393-406. PMID: 8449401

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSF Antibody (39-1) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-PSF Antibody (39-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSF Antibody (39-1) Specifications

Alternate Product Name: SFPQ

Product Clone Name: 39-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 269-362

Epitope Target: PSF

Epitope Species: human

Product Reactivity/Detection: PSF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for PSF Antibody (39-1): N/A

The preferred PSF antibody is PSF Antibody (D-8): sc-271796.