Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PSD-95 CRISPR/Cas9 KO Plasmid (m): sc-420008
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSD-95 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PSD-95 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSD-95 Antibody (7E3): sc-32290
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSD-95 CRISPR/Cas9 KO Plasmid (m) | sc-420008 | 20 µg | $397.00 |
Overview
Dlg4 encodes postsynaptic density protein 95 (PSD-95), a major MAGUK scaffold that organizes excitatory synapses by clustering NMDA- and AMPA-type glutamate receptors with signaling enzymes and cytoskeletal regulators at the postsynaptic density. PSD-95 coordinates synaptic maturation and plasticity through pathways governing receptor trafficking, calcium-dependent signaling, and activity-dependent remodeling of dendritic spines. In mouse neuroscience models, altered Dlg4/PSD-95 function is widely used to interrogate mechanisms underlying excitatory/inhibitory balance and circuit connectivity, with relevance to neurodevelopmental and neuropsychiatric phenotypes. Its central role in synaptic signaling makes it a common target for dissecting glutamatergic transmission and plasticity in vitro and in vivo.
PSD-95 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dlg4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dlg4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dlg4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PSD-95 protein expression.
This CRISPR knockout system enables efficient generation of Dlg4-deficient cell models for investigation of PSD-95 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dlg4 exon(s) critical for PSD-95 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dlg4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PSD-95 CRISPR/Cas9 KO Plasmid (m) and PSD-95 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dlg4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PSD-95 HDR Plasmid (m) and PSD-95 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dlg4 homology arms to support homology-directed repair at defined Dlg4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.