PSCA Antibody (7F5): sc-80654

PSCA Antibody (7F5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PSCA protein of human origin by western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). PSCA (7F5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Prostate stem cell antigen (PSCA) is a 123 amino acid glycosylated protein that belongs to the Thy-1/Ly-6 family of glycosyl-phosphatidylinositol (GPI)-anchored cell surface antigens, which play crucial roles in cell signaling and adhesion. PSCA gene is located on chromosome 8q24.3, where PSCA is predominantly expressed in prostate and placental tissues. Notably, PSCA is overexpressed in a significant number of prostate cancers, and PSCA transcripts are also found in high levels in urothelial tumors, indicating potential as a biomarker for both urothelial and gastric tissue carcinogenesis. The interaction of PSCA with specific proteins, such as c-myc, which is also located on chromosome 8q and is known for its role in cell proliferation and cancer progression, highlights PSCA′s importance in cancer biology. Among prostate cancer cell surface antigens, PSCA is expressed in over 80% of prostate carcinomas and is associated with certain phenotypes, including prostate cancer bone metastases, making PSCA a valuable target for therapeutic and diagnostic applications.

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PSCA Antibody (7F5) References:

1. Coamplification of prostate stem cell antigen (PSCA) and MYC in locally advanced prostate cancer.  |  Reiter, RE., et al. 2000. Genes Chromosomes Cancer. 27: 95-103. PMID: 10564591
2. Prostate stem cell antigen (PSCA) expression increases with high gleason score, advanced stage and bone metastasis in prostate cancer.  |  Gu, Z., et al. 2000. Oncogene. 19: 1288-96. PMID: 10713670
3. Reduced expression of PSCA, a member of the LY-6 family of cell surface antigens, in bladder, esophagus, and stomach tumors.  |  Bahrenberg, G., et al. 2000. Biochem Biophys Res Commun. 275: 783-8. PMID: 10973799
4. Prostate stem cell antigen is a promising candidate for immunotherapy of advanced prostate cancer.  |  Dannull, J., et al. 2000. Cancer Res. 60: 5522-8. PMID: 11034097
5. PSCA regulates IL-6 expression through p38/NF-κB signaling in prostate cancer.  |  Liu, L., et al. 2017. Prostate. 77: 1389-1400. PMID: 28845520
6. Downregulation of PSCA promotes gastric cancer proliferation and is related to poor prognosis.  |  Xu, LP., et al. 2020. J Cancer. 11: 2708-2715. PMID: 32201541
7. Significance of PSCA as a novel prognostic marker and therapeutic target for cancer.  |  Nayerpour Dizaj, T., et al. 2024. Cancer Cell Int. 24: 135. PMID: 38627732
8. Human anti-PSCA CAR macrophages possess potent antitumor activity against pancreatic cancer.  |  Shah, Z., et al. 2024. Cell Stem Cell. 31: 803-817.e6. PMID: 38663406
9. PSCA-CAR T cell therapy in metastatic castration-resistant prostate cancer: a phase 1 trial.  |  Dorff, TB., et al. 2024. Nat Med. 30: 1636-1644. PMID: 38867077
10. Potential association between PSCA rs2976395 functional variant and pancreatic cancer risk.  |  Corradi, C., et al. 2024. Int J Cancer. 155: 1432-1442. PMID: 38924078
11. Discovery of a novel highly specific, fully human PSCA antibody and its application as an antibody-drug conjugate in prostate cancer.  |  Chu, X., et al. 2024. MAbs. 16: 2387240. PMID: 39113562
12. Prostate stem cell antigen: a cell surface marker overexpressed in prostate cancer.  |  Reiter, RE., et al. 1998. Proc Natl Acad Sci U S A. 95: 1735-40. PMID: 9465086

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PSCA Antibody (7F5) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-PSCA Antibody (7F5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PSCA Antibody (7F5) Specifications

Alternate Product Name: Prostate Stem Cell Antigen

Product Clone Name: 7F5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PSCA

Epitope Species: human

Product Reactivity/Detection: PSCA

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for PSCA Antibody (7F5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin