Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRX VI Double Nickase Plasmid (h): sc-401549-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRX VI Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRX VI Double Nickase Plasmid (h) and PRX VI Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRDX6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRX VI Antibody (D-9): sc-166454
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRX VI Double Nickase Plasmid (h) | sc-401549-NIC | 20 µg | $410.00 | |||
PRX VI Double Nickase Plasmid (h2) | sc-401549-NIC-2 | 20 µg | $410.00 |
PRDX6 encodes peroxiredoxin 6 (PRX VI), a bifunctional antioxidant enzyme with glutathione peroxidase and phospholipase A2 activities that help maintain cellular redox balance and membrane phospholipid homeostasis. By limiting hydrogen peroxide and lipid hydroperoxide accumulation, PRX VI modulates oxidative stress signaling, inflammatory responses, and cell survival pathways, including processes linked to mitochondrial function and lipid metabolism. Altered PRDX6 expression or activity has been associated with dysregulated reactive oxygen species handling and lipid peroxidation, features commonly observed in inflammatory disorders, neurodegeneration, and cancer biology. As a redox and lipid repair node, PRX VI is frequently studied in models of oxidative injury, ferroptosis susceptibility, and stress-adaptive signaling.
PRX VI Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRDX6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRDX6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRDX6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRDX6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.