PRSS50 Antibody (DD-4): sc-80412

PRSS50 Antibody (DD-4) is a rat monoclonal IgG2b antibody that detects PRSS50 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PRSS50 antibody (DD-4) is available as the non-conjugated form. Testis-specific protease 50 (TSP50) is a 385 amino acid protein predominantly localized in the nucleus and cytoplasm of spermatocytes, with no expression in mature sperm or malignant mammary epithelial cells, highlighting TSP50′s critical role in spermatogenesis. The unique enzymatic structure of TSP50, which resembles serine proteases but features a threonine in place of the typical serine in the catalytic triad, suggests TSP50 may interact with specialized substrates, potentially influencing various cellular processes. TSP50 is often found to be methylated, and the presence of an abnormally hypomethylated form has been implicated in the development of breast and ovarian cancer. This indicates that while TSP50 is essential for normal reproductive function and human regeneration, TSP50′s dysregulation through hypomethylation may contribute to oncogenic pathways, making TSP50 a significant target for further research in cancer biology.

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PRSS50 Antibody (DD-4) References:

1. Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer.  |  Yuan, L., et al. 1999. Cancer Res. 59: 3215-21. PMID: 10397268
2. TSP50, a possible protease in human testes, is activated in breast cancer epithelial cells.  |  Shan, J., et al. 2002. Cancer Res. 62: 290-4. PMID: 11782390
3. Localization and expression of TSP50 protein in human and rodent testes.  |  Xu, HP., et al. 2004. Urology. 64: 826-32. PMID: 15491742
4. TESSP-1: a novel serine protease gene expressed in the spermatogonia and spermatocytes of adult mouse testes.  |  Takano, N., et al. 2005. Mol Reprod Dev. 70: 1-10. PMID: 15515062
5. Genomic and expression profiling of human spermatocytic seminomas: primary spermatocyte as tumorigenic precursor and DMRT1 as candidate chromosome 9 gene.  |  Looijenga, LH., et al. 2006. Cancer Res. 66: 290-302. PMID: 16397242
6. Screening of single-chain variable fragments against TSP50 from a phage display antibody library and their expression as soluble proteins.  |  Wei, J., et al. 2006. J Biomol Screen. 11: 546-52. PMID: 16928985
7. The male germ cell gene regulator CTCFL is functionally different from CTCF and binds CTCF-like consensus sites in a nucleosome composition-dependent manner.  |  Sleutels, F., et al. 2012. Epigenetics Chromatin. 5: 8. PMID: 22709888
8. Expression and effects of TSP50 in mouse embryo and cardiac myocyte development.  |  Ai, HH., et al. 2018. Biochem Biophys Res Commun. 502: 283-288. PMID: 29842883
9. PRSS50 is a testis protease responsible for proper sperm tail formation and function.  |  Scovell, JM., et al. 2021. Development. 148: PMID: 33913480
10. TSP50 Attenuates DSS-Induced Colitis by Regulating TGF-β Signaling Mediated Maintenance of Intestinal Mucosal Barrier Integrity.  |  Li, J., et al. 2024. Adv Sci (Weinh). 11: e2305893. PMID: 38189580
11. TSP50 facilitates breast cancer stem cell-like properties maintenance and epithelial-mesenchymal transition via PI3K p110α mediated activation of AKT signaling pathway.  |  Gao, F., et al. 2024. J Exp Clin Cancer Res. 43: 201. PMID: 39030572
12. PRSS50-mediated inhibition of MKP3/ERK signaling is crucial for meiotic progression and sperm quality.  |  Niu, CX., et al. 2024. Zool Res. 45: 1037-1047. PMID: 39147718

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRSS50 Antibody (DD-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PRSS50 Antibody (DD-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRSS50 Antibody (DD-4) Specifications

Alternate Product Name: PRSS50

Product Clone Name: DD-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: PRSS50

Epitope Species: mouse

Product Reactivity/Detection: PRSS50

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and protein stabilizer

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PRSS50 Antibody (DD-4): N/A