PRRG1 CRISPR/Cas9 KO Plasmid (h): sc-416703
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRRG1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRRG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRRG1 CRISPR/Cas9 KO Plasmid (h) | sc-416703 | 20 µg | $397.00 |
Overview
PRRG1 (proline rich and Gla domain 1) encodes a transmembrane protein characterized by an extracellular γ-carboxyglutamic acid (Gla) domain and a cytoplasmic proline-rich region consistent with protein–protein interaction and signaling scaffold functions. Although its mechanistic roles remain incompletely defined, PRRG1 has been linked to regulation of cell–cell communication and receptor-associated signaling networks that influence adhesion, cytoskeletal organization, and context-dependent cellular differentiation programs. Expression changes have been reported across multiple biological contexts, supporting use of PRRG1 as a target for investigating pathway remodeling in disease-relevant models. Functional interrogation of PRRG1 can help clarify how Gla-domain membrane proteins contribute to signaling integration and phenotypic stability in human cells.
PRRG1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRRG1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRRG1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRRG1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRRG1 protein expression.
This CRISPR knockout system enables efficient generation of PRRG1-deficient cell models for investigation of PRRG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRRG1 exon(s) critical for PRRG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRRG1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRRG1 CRISPR/Cas9 KO Plasmid (h) and PRRG1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRRG1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRRG1 HDR Plasmid (h) and PRRG1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRRG1 homology arms to support homology-directed repair at defined PRRG1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.