Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRPS2 CRISPR/Cas9 KO Plasmid (h): sc-404095
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRPS2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRPS2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRPS2 Antibody (4C11): sc-134425
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRPS2 CRISPR/Cas9 KO Plasmid (h) | sc-404095 | 20 µg | $397.00 |
Overview
PRPS2 (phosphoribosyl pyrophosphate synthetase 2) is a cytosolic enzyme that catalyzes synthesis of 5-phosphoribosyl-1-pyrophosphate (PRPP), a key metabolite required for de novo and salvage purine and pyrimidine nucleotide biosynthesis. By controlling PRPP availability, PRPS2 links ribose phosphate flux to DNA/RNA synthesis, nucleotide-dependent signaling, and cellular proliferation, integrating inputs from glycolysis and the pentose phosphate pathway. Dysregulated nucleotide metabolism and PRPP homeostasis are associated with altered growth control and metabolic rewiring observed in multiple disease contexts, including cancer and inherited purine pathway disorders. PRPS2 is therefore widely studied in mechanisms of nucleotide stress, replication dynamics, and metabolic vulnerabilities in human cells.
PRPS2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRPS2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRPS2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRPS2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRPS2 protein expression.
This CRISPR knockout system enables efficient generation of PRPS2-deficient cell models for investigation of PRPS2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRPS2 exon(s) critical for PRPS2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRPS2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRPS2 CRISPR/Cas9 KO Plasmid (h) and PRPS2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRPS2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRPS2 HDR Plasmid (h) and PRPS2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRPS2 homology arms to support homology-directed repair at defined PRPS2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.