PrP Antibody (8B4): sc-47729

PrP Antibody (8B4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the prion protein (PrP ) of mammalian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). PrP (8B4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The prion protein plays a crucial role in neuroprotection and cellular signaling, and misfolding is central to the pathogenesis of prion diseases, which are fatal neurodegenerative disorders. The normal cellular form of PrP (PrPc) is predominantly located in the brain, where PrPc is involved in synaptic function and neuroprotection. However, when PrPc undergoes conformational changes to form the disease-associated isoform (PrPSc), PrPSc becomes resistant to proteolytic degradation, leading to the accumulation of toxic aggregates that disrupt cellular function and trigger neurodegeneration. The presence of PrPSc in the brains of affected animals, such as those with scrapie, bovine spongiform encephalopathy, and Creutzfeldt-Jakob disease, highlights the importance of monitoring PrP levels and conformations in research and diagnostic applications. Anti-PrP antibody (8B4) is an essential tool for studying these critical processes and understanding the mechanisms underlying prion diseases.

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PrP Antibody (8B4) References:

1. Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agent.  |  Bessen, RA. and Marsh, RF. 1992. J Virol. 66: 2096-101. PMID: 1347795
2. Platelet-rich plasma (PRP) for knee disorders.  |  Shahid, M. and Kundra, R. 2017. EFORT Open Rev. 2: 28-34. PMID: 28607768
3. PRP and BMAC for Musculoskeletal Conditions via Biomaterial Carriers.  |  Yamaguchi, FSM., et al. 2019. Int J Mol Sci. 20: PMID: 31717698
4. A narrative review of platelet-rich plasma (PRP) in reproductive medicine.  |  Sharara, FI., et al. 2021. J Assist Reprod Genet. 38: 1003-1012. PMID: 33723748
5. Ovarian response to intraovarian platelet-rich plasma (PRP) administration: hypotheses and potential mechanisms of action.  |  Seckin, S., et al. 2022. J Assist Reprod Genet. 39: 37-61. PMID: 35175511
6. Application of platelet-rich plasma (PRP) in lips rejuvenation.  |  Huang, Y., et al. 2023. Head Face Med. 19: 24. PMID: 37365609
7. Creatine Promotes Endometriosis by Inducing Ferroptosis Resistance via Suppression of PrP.  |  Chen, S., et al. 2024. Adv Sci (Weinh). 11: e2403517. PMID: 39119937
8. Non-genetic propagation of strain-specific properties of scrapie prion protein.  |  Bessen, RA., et al. 1995. Nature. 375: 698-700. PMID: 7791905
9. Recombinant prion protein rPrP27-30 from Syrian golden hamster reveals proteinase K sensitivity.  |  Weiss, S., et al. 1996. Biochem Biophys Res Commun. 219: 173-9. PMID: 8619803
10. Complete genomic sequence and analysis of the prion protein gene region from three mammalian species.  |  Lee, IY., et al. 1998. Genome Res. 8: 1022-37. PMID: 9799790
11. Prions.  |  Prusiner, SB. 1998. Proc Natl Acad Sci U S A. 95: 13363-83. PMID: 9811807
12. Strain-dependent differences in beta-sheet conformations of abnormal prion protein.  |  Caughey, B., et al. 1998. J Biol Chem. 273: 32230-5. PMID: 9822701

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PrP Antibody (8B4) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-PrP Antibody (8B4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PrP Antibody (8B4) Specifications

Alternate Product Name: PrP

Product Clone Name: 8B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PrP

Epitope Species: mouse

Product Reactivity/Detection: PrP

Species Reactivity/Detection: mammalian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for PrP Antibody (8B4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin