protein 4.2 CRISPR/Cas9 KO Plasmid (h): sc-406824
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- protein 4.2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the protein 4.2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
protein 4.2 CRISPR/Cas9 KO Plasmid (h) | sc-406824 | 20 µg | $397.00 |
Overview
EPB42 encodes erythrocyte membrane protein 4.2, a cytoskeletal adaptor that stabilizes the red blood cell membrane by coupling the band 3 (SLC4A1) complex to spectrin–actin junctional networks. Protein 4.2 supports membrane cohesion, biconcave cell shape, and mechanical resilience during microvascular transit, integrating into pathways that govern membrane organization and ion transport homeostasis. Disruption of EPB42 perturbs erythrocyte membrane integrity and is associated with red cell fragility phenotypes observed in hereditary hemolytic disorders, providing a mechanistic link between cytoskeletal assembly and hemolysis-related pathophysiology. This gene is therefore widely used to interrogate membrane–cytoskeleton interactions, protein complex stoichiometry, and stress responses in erythroid biology.
protein 4.2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EPB42 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EPB42 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EPB42 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish protein 4.2 protein expression.
This CRISPR knockout system enables efficient generation of EPB42-deficient cell models for investigation of protein 4.2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EPB42 exon(s) critical for protein 4.2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EPB42 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by protein 4.2 CRISPR/Cas9 KO Plasmid (h) and protein 4.2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EPB42 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by protein 4.2 HDR Plasmid (h) and protein 4.2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EPB42 homology arms to support homology-directed repair at defined EPB42 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.