Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Prostein Double Nickase Plasmid (h): sc-406577-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Prostein Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Prostein Double Nickase Plasmid (h) and Prostein Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC45A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Prostein Antibody (A-5): sc-393069
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Prostein Double Nickase Plasmid (h) | sc-406577-NIC | 20 µg | $410.00 | |||
Prostein Double Nickase Plasmid (h2) | sc-406577-NIC-2 | 20 µg | $410.00 |
SLC45A3 encodes prostein, a prostate-enriched multi-pass membrane protein that localizes predominantly to the Golgi apparatus and contributes to epithelial secretory and trafficking processes. Its expression is regulated by androgen receptor signaling and is commonly used as a molecular marker of prostate lineage and differentiation status. Altered SLC45A3 expression patterns and recurrent gene rearrangements involving the SLC45A3 locus have been described in prostate cancer biology, supporting its utility in studying androgen-responsive transcriptional programs. Functional perturbation of prostein provides a tractable route to interrogate membrane trafficking, Golgi homeostasis, and lineage-associated regulatory networks in human prostate-derived model systems.
Prostein Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC45A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC45A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC45A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC45A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.