Prostasin Antibody (C-10): sc-514983
- Prostasin Antibody (C-10) is a mouse monoclonal IgG2a κ Prostasin antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 200-228 within an internal region of Prostasin of mouse origin
- Prostasin Antibody (C-10) is recommended for detection of precursor and mature heavy chain Prostasin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Prostasin Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Prostasin Antibody (C-10). This work is in progress.
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Prostasin Antibody (C-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects Prostasin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Prostasin antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Prostasin, a serine protease primarily identified in prostate tissue, plays a crucial role in activating epithelial sodium channels across various tissues, which is essential for maintaining sodium balance and fluid homeostasis in the body. While typically anchored to the cell membrane, Prostasin can also be found in physiological fluids and tissue culture media, suggesting a potential mechanism for secretion from cells. This secretion is significant as Prostasin may influence local tissue environments and cellular interactions. Additionally, Prostasin′s activity is modulated by protease inhibitors like aprotinin, which can suppress channel-activating capacity, while aldosterone enhances expression and promotes sodium uptake. Notably, DNA methylation has been shown to negatively correlate with Prostasin expression in cancer cells, and enforced reexpression can reduce invasiveness and metastatic potential, highlighting Prostasin′s importance in epithelial cell physiology and cancer progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Prostasin Antibody (C-10) References:
- Prostasin serine protease inhibits breast cancer invasiveness and is transcriptionally regulated by promoter DNA methylation. | Chen, LM. and Chai, KX. 2002. Int J Cancer. 97: 323-9. PMID: 11774283
- Regulation of prostasin by aldosterone in the kidney. | Narikiyo, T., et al. 2002. J Clin Invest. 109: 401-8. PMID: 11828000
- Serine protease activity in m-1 cortical collecting duct cells. | Liu, L., et al. 2002. Hypertension. 39: 860-4. PMID: 11967240
- Inhibition of prostasin secretion by serine protease inhibitors in the kidney. | Iwashita, K., et al. 2003. J Am Soc Nephrol. 14: 11-6. PMID: 12506133
- Adenovirus-mediated human prostasin gene delivery is linked to increased aldosterone production and hypertension in rats. | Wang, C., et al. 2003. Am J Physiol Regul Integr Comp Physiol. 284: R1031-6. PMID: 12626364
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Prostasin Antibody (C-10) | sc-514983 | 200 µg/ml | $316.00 | |||
Prostasin Antibody (C-10) AC | sc-514983 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Prostasin Antibody (C-10) HRP | sc-514983 HRP | 200 µg/ml | $316.00 | |||
Prostasin Antibody (C-10) FITC | sc-514983 FITC | 200 µg/ml | $330.00 | |||
Prostasin Antibody (C-10) PE | sc-514983 PE | 200 µg/ml | $343.00 | |||
Prostasin Antibody (C-10) Alexa Fluor® 488 | sc-514983 AF488 | 200 µg/ml | $357.00 | |||
Prostasin Antibody (C-10) Alexa Fluor® 546 | sc-514983 AF546 | 200 µg/ml | $357.00 | |||
Prostasin Antibody (C-10) Alexa Fluor® 594 | sc-514983 AF594 | 200 µg/ml | $357.00 | |||
Prostasin Antibody (C-10) Alexa Fluor® 647 | sc-514983 AF647 | 200 µg/ml | $357.00 | |||
Prostasin Antibody (C-10) Alexa Fluor® 680 | sc-514983 AF680 | 200 µg/ml | $357.00 | |||
Prostasin Antibody (C-10) Alexa Fluor® 790 | sc-514983 AF790 | 200 µg/ml | $357.00 | |||
Prostasin (C-10) Neutralizing Peptide | sc-514983 P | 100 µg/0.5 ml | $68.00 |